A highly sensitive colorimetric determination of serum zinc using water-soluble pyridylazo dye. Export

@article{citeulike:4156789, abstract = {A colorimetric method for precise and accurate determination of zinc in serum is presented. Only 0.3 ml of sample is necessary, because of the use of a new, highly sensitive reagent, 2-(5-bromo-2-pyridylazo)-5-(N-n-propyl-N-3-sulfopropylamino)-phenol (epsilon 554nm = 1.3 X 10(5) 1 . mol-1 . cm-1), which is water-soluble and has long-term stability. Interference of iron and copper in serum can be removed by co-precipitation of the iron fluoride complex with trichloroacetic acid precipitated proteins and the copper dithiocarboxy sarcosine complex, respectively. Within-run and day-to-day precision (CV) are in the range of 0.3-3.5\% and 1.9-3.1\%, respectively, depending on the serum zinc content. A good correlation (r = 0.98, p less than 0.05) was obtained between this method and atomic absorption spectrometry. In contrast to previous colorimetric methods, the present method does not involve heating, extraction with organic solvents, or a cyanide masking system.}, author = {Makino, T. and Saito, M. and Horiguchi, D. and Kina, K.}, citeulike-article-id = {4156789}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/7067132}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=7067132}, day = {26}, issn = {0009-8981}, journal = {Clinica chimica acta; international journal of clinical chemistry}, keywords = {assay, serum}, month = {March}, number = {1}, pages = {127--135}, posted-at = {2009-03-10 07:03:09}, priority = {2}, title = {A highly sensitive colorimetric determination of serum zinc using water-soluble pyridylazo dye.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/7067132}, volume = {120}, year = {1982} }

TY - JOUR ID - citeulike:4156789 L3 - citeulike-article-id:4156789 N2 - A colorimetric method for precise and accurate determination of zinc in serum is presented. Only 0.3 ml of sample is necessary, because of the use of a new, highly sensitive reagent, 2-(5-bromo-2-pyridylazo)-5-(N-n-propyl-N-3-sulfopropylamino)-phenol (epsilon 554nm = 1.3 X 10(5) 1 . mol-1 . cm-1), which is water-soluble and has long-term stability. Interference of iron and copper in serum can be removed by co-precipitation of the iron fluoride complex with trichloroacetic acid precipitated proteins and the copper dithiocarboxy sarcosine complex, respectively. Within-run and day-to-day precision (CV) are in the range of 0.3-3.5% and 1.9-3.1%, respectively, depending on the serum zinc content. A good correlation (r = 0.98, p less than 0.05) was obtained between this method and atomic absorption spectrometry. In contrast to previous colorimetric methods, the present method does not involve heating, extraction with organic solvents, or a cyanide masking system. IS - 1 JF - Clinica chimica acta; international journal of clinical chemistry SN - 0009-8981 EP - 135 TI - A highly sensitive colorimetric determination of serum zinc using water-soluble pyridylazo dye. VL - 120 SP - 127 KW - assay KW - serum AU - Makino, T AU - Saito, M AU - Horiguchi, D AU - Kina, K PY - 1982/03/26/ UR - http://view.ncbi.nlm.nih.gov/pubmed/7067132 ER -