Establishment of two human prostate cancer cell lines derived from a single bone metastasis. Export

@article{citeulike:5026396, abstract = {Human prostate cancer cell lines are particularly difficult to establish, and most existing cell lines do not exhibit features commonly seen in human prostate cancer. Most available models either grow only in vivo as xenografts or are androgen insensitive and fail to express prostate-specific antigen (PSA). The lack of functionally relevant model systems of advanced prostate cancer has limited prostate cancer research and therapy development. Of 30 processed samples derived from patients with prostate cancer, we established two cell lines (MDA PCa 2a and MDA PCa 2b) that express PSA and androgen receptor, grow in vitro, and are androgen sensitive. Cells from these lines produced tumors in nude mice when injected either s. c. or orthotopically (intraprostatic). Both cell lines were established from a bone metastasis of a patient whose cancer was exhibiting androgen-independent growth. Although both were derived from two samples of the same specimen, they have different genetic features (as assessed by karyotype analysis) and different phenotypes (e.g., morphology and growth rate). It is likely that they are distinct clones isolated by the use of different culture procedures and reflect the genetic heterogeneity of the tumor. These new cell lines are the first available derived from a bone metastasis of an androgen-independent prostatic adenocarcinoma that grow both in vivo and in vitro and have retained PSA expression and androgen sensitivity. They therefore constitute important model systems to address critical questions related to the androgen-independent growth of human prostate cancer and to the complex process of bone metastasis.}, author = {Navone, N. M. and Olive, M. and Ozen, M. and Davis, R. and Troncoso, P. and Tu, S. M. and Johnston, D. and Pollack, A. and Pathak, S. and von Eschenbach, A. C. and Logothetis, C. J.}, citeulike-article-id = {5026396}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/9815652}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=9815652}, issn = {1078-0432}, journal = {Clinical cancer research : an official journal of the American Association for Cancer Research}, keywords = {cell-lines, historical}, month = {December}, number = {12 Pt 1}, pages = {2493--2500}, posted-at = {2009-06-30 20:56:06}, priority = {2}, title = {Establishment of two human prostate cancer cell lines derived from a single bone metastasis.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/9815652}, volume = {3}, year = {1997} }

TY - JOUR ID - citeulike:5026396 L3 - citeulike-article-id:5026396 N2 - Human prostate cancer cell lines are particularly difficult to establish, and most existing cell lines do not exhibit features commonly seen in human prostate cancer. Most available models either grow only in vivo as xenografts or are androgen insensitive and fail to express prostate-specific antigen (PSA). The lack of functionally relevant model systems of advanced prostate cancer has limited prostate cancer research and therapy development. Of 30 processed samples derived from patients with prostate cancer, we established two cell lines (MDA PCa 2a and MDA PCa 2b) that express PSA and androgen receptor, grow in vitro, and are androgen sensitive. Cells from these lines produced tumors in nude mice when injected either s. c. or orthotopically (intraprostatic). Both cell lines were established from a bone metastasis of a patient whose cancer was exhibiting androgen-independent growth. Although both were derived from two samples of the same specimen, they have different genetic features (as assessed by karyotype analysis) and different phenotypes (e.g., morphology and growth rate). It is likely that they are distinct clones isolated by the use of different culture procedures and reflect the genetic heterogeneity of the tumor. These new cell lines are the first available derived from a bone metastasis of an androgen-independent prostatic adenocarcinoma that grow both in vivo and in vitro and have retained PSA expression and androgen sensitivity. They therefore constitute important model systems to address critical questions related to the androgen-independent growth of human prostate cancer and to the complex process of bone metastasis. IS - 12 Pt 1 JF - Clinical cancer research : an official journal of the American Association for Cancer Research SN - 1078-0432 EP - 2500 TI - Establishment of two human prostate cancer cell lines derived from a single bone metastasis. VL - 3 SP - 2493 KW - cell-lines KW - historical AU - Navone, NM AU - Olive, M AU - Ozen, M AU - Davis, R AU - Troncoso, P AU - Tu, SM AU - Johnston, D AU - Pollack, A AU - Pathak, S AU - von Eschenbach, AC AU - Logothetis, CJ PY - 1997/12// UR - http://view.ncbi.nlm.nih.gov/pubmed/9815652 ER -