In vivo BOLD contrast MRI mapping of subcutaneous vascular function and maturation: Validation by intravital microscopy Export

@article{citeulike:4308382, abstract = {Bold contrast MRI was applied for mapping vascular maturation in tumor- and wound-induced skin angiogenesis using the response of mature vessels to hypercapnia (inhalation of air vs. air 5\% CO2) and the response of all vessels to hyperoxia (air 5\% CO2 vs. oxygen 5\% CO2 (carbogen)). MRI signal enhancement with hypercapnia was reduced in centered vs. linear phase encoding, suggesting increased blood flow. However, intravital microscopy demonstrated constriction of arterioles and reduced flux and density of red blood cells in mature capillaries with hypercapnia, with no change in the diameter of wound-induced neovasculature. The discrepancy in flow between MRI and intravital microscopy is consistent with increased plasma flow and reduced hematocrit. Hyperoxia resulted in increased blood oxygenation and constriction of all vessels. These results provide a hemodynamic explanation for the selective registration of MRI response to hypercapnia with mature vessels and the response to hyperoxia with total vascular function. Magn Reson Med 45:887-898, 2001. {\copyright} 2001 Wiley-Liss, Inc.}, address = {Department of Biological Regulation, The Weizmann Institute of Science, Rehovot, Israel; Radiation Oncology, Duke University Medical Center, Durham, North Carolina}, author = {Neeman, Michal and Dafni, Hagit and Bukhari, Omar and Braun, Rod D. and Dewhirst, Mark W.}, citeulike-article-id = {4308382}, citeulike-linkout-0 = {http://dx.doi.org/10.1002/mrm.1118}, citeulike-linkout-1 = {http://www3.interscience.wiley.com/cgi-bin/abstract/79502166/ABSTRACT}, doi = {10.1002/mrm.1118}, journal = {Magnetic Resonance in Medicine}, keywords = {animal-models, bold, intravital-microscopy, mri, tumor}, number = {5}, pages = {887--898}, posted-at = {2009-04-14 20:17:01}, priority = {2}, title = {In vivo BOLD contrast MRI mapping of subcutaneous vascular function and maturation: Validation by intravital microscopy}, url = {http://dx.doi.org/10.1002/mrm.1118}, volume = {45}, year = {2001} }

TY - JOUR ID - citeulike:4308382 L3 - citeulike-article-id:4308382 N2 - Bold contrast MRI was applied for mapping vascular maturation in tumor- and wound-induced skin angiogenesis using the response of mature vessels to hypercapnia (inhalation of air vs. air 5% CO2) and the response of all vessels to hyperoxia (air 5% CO2 vs. oxygen 5% CO2 (carbogen)). MRI signal enhancement with hypercapnia was reduced in centered vs. linear phase encoding, suggesting increased blood flow. However, intravital microscopy demonstrated constriction of arterioles and reduced flux and density of red blood cells in mature capillaries with hypercapnia, with no change in the diameter of wound-induced neovasculature. The discrepancy in flow between MRI and intravital microscopy is consistent with increased plasma flow and reduced hematocrit. Hyperoxia resulted in increased blood oxygenation and constriction of all vessels. These results provide a hemodynamic explanation for the selective registration of MRI response to hypercapnia with mature vessels and the response to hyperoxia with total vascular function. Magn Reson Med 45:887-898, 2001. © 2001 Wiley-Liss, Inc. IS - 5 JF - Magnetic Resonance in Medicine AD - Department of Biological Regulation, The Weizmann Institute of Science, Rehovot, Israel; Radiation Oncology, Duke University Medical Center, Durham, North Carolina EP - 898 TI - In vivo BOLD contrast MRI mapping of subcutaneous vascular function and maturation: Validation by intravital microscopy VL - 45 SP - 887 KW - animal-models KW - bold KW - intravital-microscopy KW - mri KW - tumor AU - Neeman, Michal AU - Dafni, Hagit AU - Bukhari, Omar AU - Braun, Rod AU - Dewhirst, Mark PY - 2001/// UR - http://dx.doi.org/10.1002/mrm.1118 ER -