Differential CD146 Expression on Circulating Versus Tissue Endothelial Cells in Rectal Cancer Patients: Implications for Circulating Endothelial and Progenitor Cells As Biomarkers for Antiangiogenic Therapy Export

@article{citeulike:4311940, abstract = {PURPOSE: Circulating endothelial cells (CECs) and progenitor cells are currently evaluated as potential biomarkers of antiangiogenic therapy. CD146 is considered a panendothelial-specific marker, but its utility as a CEC marker in cancer patients remains unclear. PATIENTS AND METHODS: We analyzed the expression of CD146 on mononuclear blood cells, primary tissue endothelial cells, and malignant and normal tissues by flow cytometric and immunohistochemical analyses. Furthermore, we measured the circulation kinetics of CD146+ cells before, and then 3 and 12 days after vascular endothelial growth factor (VEGF) antibody blockade by bevacizumab infusion in rectal cancer patients enrolled in a phase I trial. RESULTS: In the peripheral blood of these cancer patients, over 90\% of the CD146+ cells were CD45+ hematopoietic cells. CD146 expression was primarily detected on a subset of CD3+CD4+ lymphocytes, and was undetectable on CD34+CD133+CD45dim progenitor cells or CD31brightCD45- viable CECs. In contradistinction, CD146 was detectable in tissues on both cellular components of tumor vessel wall: CD31brightCD45- endothelial cells and {alpha}-SMA+ pericytes. Unlike viable CECs and progenitor cells, CD146+ cell concentration in the peripheral blood of cancer patients did not decrease during VEGF blockade. CONCLUSION: CD146 is fairly homogeneously expressed on vascular endothelium but not on viable CECs or progenitor cells. The vast majority of CD146+ blood cells are lymphocytes, and VEGF blockade by bevacizumab did not significantly change their number in rectal cancer patients. These results underscore the need for further characterization and identification of new markers for CEC subpopulations for their development as biomarkers of antiangiogenic therapy. 10.1200/JCO.2005.04.2861}, author = {Duda, Dan G. and Cohen, Kenneth S. and di Tomaso, Emmanuelle and Au, Patrick and Klein, Rachael J. and Scadden, David T. and Willett, Christopher G. and Jain, Rakesh K.}, citeulike-article-id = {4311940}, citeulike-linkout-0 = {http://dx.doi.org/10.1200/JCO.2005.04.2861}, citeulike-linkout-1 = {http://jco.ascopubs.org/cgi/content/abstract/24/9/1449}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/16549839}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=16549839}, day = {20}, doi = {10.1200/JCO.2005.04.2861}, journal = {J Clin Oncol}, keywords = {antiangiogenic, biomarker, syn\_ai\_042009}, month = {March}, number = {9}, pages = {1449--1453}, posted-at = {2009-04-15 09:54:22}, priority = {2}, title = {Differential CD146 Expression on Circulating Versus Tissue Endothelial Cells in Rectal Cancer Patients: Implications for Circulating Endothelial and Progenitor Cells As Biomarkers for Antiangiogenic Therapy}, url = {http://dx.doi.org/10.1200/JCO.2005.04.2861}, volume = {24}, year = {2006} }

TY - JOUR ID - citeulike:4311940 L3 - citeulike-article-id:4311940 N2 - PURPOSE: Circulating endothelial cells (CECs) and progenitor cells are currently evaluated as potential biomarkers of antiangiogenic therapy. CD146 is considered a panendothelial-specific marker, but its utility as a CEC marker in cancer patients remains unclear. PATIENTS AND METHODS: We analyzed the expression of CD146 on mononuclear blood cells, primary tissue endothelial cells, and malignant and normal tissues by flow cytometric and immunohistochemical analyses. Furthermore, we measured the circulation kinetics of CD146+ cells before, and then 3 and 12 days after vascular endothelial growth factor (VEGF) antibody blockade by bevacizumab infusion in rectal cancer patients enrolled in a phase I trial. RESULTS: In the peripheral blood of these cancer patients, over 90% of the CD146+ cells were CD45+ hematopoietic cells. CD146 expression was primarily detected on a subset of CD3+CD4+ lymphocytes, and was undetectable on CD34+CD133+CD45dim progenitor cells or CD31brightCD45- viable CECs. In contradistinction, CD146 was detectable in tissues on both cellular components of tumor vessel wall: CD31brightCD45- endothelial cells and {alpha}-SMA+ pericytes. Unlike viable CECs and progenitor cells, CD146+ cell concentration in the peripheral blood of cancer patients did not decrease during VEGF blockade. CONCLUSION: CD146 is fairly homogeneously expressed on vascular endothelium but not on viable CECs or progenitor cells. The vast majority of CD146+ blood cells are lymphocytes, and VEGF blockade by bevacizumab did not significantly change their number in rectal cancer patients. These results underscore the need for further characterization and identification of new markers for CEC subpopulations for their development as biomarkers of antiangiogenic therapy. 10.1200/JCO.2005.04.2861 IS - 9 JF - J Clin Oncol EP - 1453 TI - Differential CD146 Expression on Circulating Versus Tissue Endothelial Cells in Rectal Cancer Patients: Implications for Circulating Endothelial and Progenitor Cells As Biomarkers for Antiangiogenic Therapy VL - 24 SP - 1449 KW - antiangiogenic KW - biomarker KW - syn_ai_042009 AU - Duda, Dan AU - Cohen, Kenneth AU - di Tomaso, Emmanuelle AU - Au, Patrick AU - Klein, Rachael AU - Scadden, David AU - Willett, Christopher AU - Jain, Rakesh PY - 2006/03/20/ UR - http://dx.doi.org/10.1200/JCO.2005.04.2861 ER -