Exogenous mesenchymal stem cells localize to the kidney by means of CD44 following acute tubular injury. Export

@article{citeulike:1718174, abstract = {Mesenchymal stem cells (MSC) were recently shown to migrate to injured tissues when transplanted systemically. The mechanisms underlying the migration and homing of these cells is, however, unclear. In this study, we examine the role of CD44 and its major ligand, hyaluronic acid, in the trafficking of intravenously injected MSC in the glycerol-induced mouse model of acute renal failure (ARF). In vitro, hyaluronic acid promoted a dose-dependent migration of the stem cells that was inhibited by an anti-CD44 blocking monoclonal antibody. In vivo, stem cells injected into mice with ARF migrated to the injured kidney where hyaluronic acid expression was increased. Their presence correlated with morphological and functional recovery. Renal localization of the MSC was blocked by pre-incubation with the CD44 blocking antibody or by soluble hyaluronic acid. Stem cells derived from CD44 knockout mice did not localize to the injured kidney and did not accelerate morphological or functional recovery. Reconstitution by transfection of CD44 knockout stem cells with cDNA encoding wild-type CD44, but not a loss of function CD44 unable to bind hyaluronic acid, restored in vitro migration and in vivo localization of the cells to injured kidneys. We suggest that CD44 and hyaluronic acid interactions recruit exogenous MSC to injured renal tissue and enhance renal regeneration.}, address = {Department of Internal Medicine, University of Torino, Torino, Italy.}, author = {Herrera, M. B. and Bussolati, B. and Bruno, S. and Morando, L. and Mauriello-Romanazzi, G. and Sanavio, F. and Stamenkovic, I. and Biancone, L. and Camussi, G.}, citeulike-article-id = {1718174}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/sj.ki.5002334}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/17507906}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=17507906}, doi = {10.1038/sj.ki.5002334}, issn = {0085-2538}, journal = {Kidney Int}, keywords = {aki, cd44, kidney, stem\_cell}, month = {August}, number = {4}, pages = {430--441}, posted-at = {2007-12-26 13:51:56}, priority = {2}, title = {Exogenous mesenchymal stem cells localize to the kidney by means of CD44 following acute tubular injury.}, url = {http://dx.doi.org/10.1038/sj.ki.5002334}, volume = {72}, year = {2007} }

TY - JOUR ID - citeulike:1718174 L3 - citeulike-article-id:1718174 N2 - Mesenchymal stem cells (MSC) were recently shown to migrate to injured tissues when transplanted systemically. The mechanisms underlying the migration and homing of these cells is, however, unclear. In this study, we examine the role of CD44 and its major ligand, hyaluronic acid, in the trafficking of intravenously injected MSC in the glycerol-induced mouse model of acute renal failure (ARF). In vitro, hyaluronic acid promoted a dose-dependent migration of the stem cells that was inhibited by an anti-CD44 blocking monoclonal antibody. In vivo, stem cells injected into mice with ARF migrated to the injured kidney where hyaluronic acid expression was increased. Their presence correlated with morphological and functional recovery. Renal localization of the MSC was blocked by pre-incubation with the CD44 blocking antibody or by soluble hyaluronic acid. Stem cells derived from CD44 knockout mice did not localize to the injured kidney and did not accelerate morphological or functional recovery. Reconstitution by transfection of CD44 knockout stem cells with cDNA encoding wild-type CD44, but not a loss of function CD44 unable to bind hyaluronic acid, restored in vitro migration and in vivo localization of the cells to injured kidneys. We suggest that CD44 and hyaluronic acid interactions recruit exogenous MSC to injured renal tissue and enhance renal regeneration. IS - 4 JF - Kidney Int SN - 0085-2538 AD - Department of Internal Medicine, University of Torino, Torino, Italy. EP - 441 TI - Exogenous mesenchymal stem cells localize to the kidney by means of CD44 following acute tubular injury. VL - 72 SP - 430 KW - aki KW - cd44 KW - kidney KW - stem_cell AU - Herrera, MB AU - Bussolati, B AU - Bruno, S AU - Morando, L AU - Mauriello-Romanazzi, G AU - Sanavio, F AU - Stamenkovic, I AU - Biancone, L AU - Camussi, G PY - 2007/08// UR - http://dx.doi.org/10.1038/sj.ki.5002334 ER -