Hepatocytes enhance effects of lipopolysaccharide on liver nonparenchymal cells through close cell interactions Export

@article{Scott:2005p553, abstract = {The response to lipopolysaccharide (LPS) in the liver is complex, requiring cell-to-cell interactions between hepatocytes and liver nonparenchymal cells (NPC), in particular, Kupffer cells. Previous studies show that cytokines produced by Kupffer cells stimulated with LPS can, in turn, activate hepatocytes. In the present study, we sought to examine whether the reverse, hepatocyte (HC)-NPC interactions, is important in cytokine production in mixed cell cocultures. LPS-stimulated production of tumor necrosis factor (TNF)-alpha and interleukin (IL)-6 from NPC was augmented in mixed HC-NPC cocultures, as compared with NPC monocultures. This HC-NPC interaction was not observed when hepatocytes were cocultured with NPC from TLR4-mutant (C3H/HeJ) mice or CD14-deficient mice. The effect was partially lost when hepatocytes from lipopolysaccharide-binding protein (LBP)-deficient mice were cocultured with wild-type mice. These data indicate that functional TLR4 and CD14 are required for NPC production of cytokines and that at least one of the critical components from hepatocytes is LBP. The augmented cytokine production by mixed HC-NPC cocultures was abrogated when the cells were separated by a filter system, indicating that close cell interactions are also required for this interaction. Thus, interaction between hepatocytes and NPC are critical for cytokine secretion by NPC.}, author = {Scott, Melanie J. and Liu, Shubing and Su, Grace L. and Vodovotz, Yoram and Billiar, Timothy R.}, citeulike-article-id = {3046174}, citeulike-linkout-0 = {http://meta.wkhealth.com/pt/pt-core/template-journal/lwwgateway/media/landingpage.htm?issn=1073-2322&volume=23&issue=5&spage=453}, journal = {Shock}, keywords = {animals, antigens, assay, binding, c3h, cd14, cell, cells, coculture, communication, cultured, cytokines, enzyme-linked, factor-alpha, factors, file-import-08-07-27, hepatocytes, immunosorbent, inbred, interleukin-6, kupffer, lipopolysaccharides, liver, mice, mutation, necrosis, protein, techniques, time, transgenic, tumor}, local-url = {file://localhost/Users/ken/Documents/Papers/2005/Scott/Shock\%202005\%20Scott.pdf}, month = {May}, number = {5}, pages = {453--8}, posted-at = {2008-07-27 14:45:57}, priority = {2}, title = {Hepatocytes enhance effects of lipopolysaccharide on liver nonparenchymal cells through close cell interactions}, url = {http://meta.wkhealth.com/pt/pt-core/template-journal/lwwgateway/media/landingpage.htm?issn=1073-2322&volume=23&issue=5&spage=453}, volume = {23}, year = {2005} }

TY - JOUR ID - Scott:2005p553 L3 - citeulike-article-id:3046174 N2 - The response to lipopolysaccharide (LPS) in the liver is complex, requiring cell-to-cell interactions between hepatocytes and liver nonparenchymal cells (NPC), in particular, Kupffer cells. Previous studies show that cytokines produced by Kupffer cells stimulated with LPS can, in turn, activate hepatocytes. In the present study, we sought to examine whether the reverse, hepatocyte (HC)-NPC interactions, is important in cytokine production in mixed cell cocultures. LPS-stimulated production of tumor necrosis factor (TNF)-alpha and interleukin (IL)-6 from NPC was augmented in mixed HC-NPC cocultures, as compared with NPC monocultures. This HC-NPC interaction was not observed when hepatocytes were cocultured with NPC from TLR4-mutant (C3H/HeJ) mice or CD14-deficient mice. The effect was partially lost when hepatocytes from lipopolysaccharide-binding protein (LBP)-deficient mice were cocultured with wild-type mice. These data indicate that functional TLR4 and CD14 are required for NPC production of cytokines and that at least one of the critical components from hepatocytes is LBP. The augmented cytokine production by mixed HC-NPC cocultures was abrogated when the cells were separated by a filter system, indicating that close cell interactions are also required for this interaction. Thus, interaction between hepatocytes and NPC are critical for cytokine secretion by NPC. IS - 5 JF - Shock EP - 8 TI - Hepatocytes enhance effects of lipopolysaccharide on liver nonparenchymal cells through close cell interactions VL - 23 SP - 453 KW - animals KW - antigens KW - assay KW - binding KW - c3h KW - cd14 KW - cell KW - cells KW - coculture KW - communication KW - cultured KW - cytokines KW - enzyme-linked KW - factor-alpha KW - factors KW - file-import-08-07-27 KW - hepatocytes KW - immunosorbent KW - inbred KW - interleukin-6 KW - kupffer KW - lipopolysaccharides KW - liver KW - mice KW - mutation KW - necrosis KW - protein KW - techniques KW - time KW - transgenic KW - tumor AU - Scott, Melanie AU - Liu, Shubing AU - Su, Grace AU - Vodovotz, Yoram AU - Billiar, Timothy PY - 2005/May// UR - http://meta.wkhealth.com/pt/pt-core/template-journal/lwwgateway/media/landingpage.htm?issn=1073-2322&volume=23&issue=5&spage=453 ER -