Palmitic acid induces IP-10 expression in human macrophages via NF-kappaB activation Export

@article{Laine:2007p722, abstract = {It is now recognized that cross-talk between adipocytes and adipose tissue stromal cells such as macrophages contributes to local and systemic inflammation. One factor from adipocytes that may participate in this interaction and that is frequently elevated in inflammatory conditions such as obesity, insulin resistance, and type 2 diabetes is free fatty acids (FFA). To investigate the potential for FFA to enhance macrophage inflammation, we exposed U937 macrophages to physiological levels (150 microM) of FFA. Palmitic acid (PA), the predominant saturated FFA released from adipose tissue, but not unsaturated FFA, induced an approximately 6-fold (p<0.05) increase in IP-10 gene expression (and 2- to 4-fold increases in IL-8, MCP-1, COX-2, and MIG). PA also induced an approximately 2-fold increase (p<0.05) in active NF-kappaB, and two structurally distinct NF-kappaB inhibitors effectively blocked PA-induced IP-10 gene expression. Conditioned medium from PA-treated cells increased lymphocyte migration 41\% (p<0.05) which was significantly reduced by IP-10-neutralizing antibody. These results suggest that elevated concentrations of PA commonly present in obese and insulin resistant individuals can increase NF-kappaB-mediated expression of IP-10 in macrophages. These events in turn may lead to an increasing feed-forward loop of chronic inflammation.}, author = {Laine, Phyllis S. and Schwartz, Eric A. and Wang, Yingjie and Zhang, Wei Y. and Karnik, Sheetal K. and Musi, Nicolas and Reaven, Peter D.}, citeulike-article-id = {3046185}, citeulike-linkout-0 = {http://www.sciencedirect.com/science?_ob=ArticleURL&#38;_udi=B6WBK-4NJ25B7-B&#38;_user=1029198&#38;_rdoc=1&#38;_fmt=&#38;_orig=search&#38;_sort=d&#38;view=c&#38;_acct=C000050560&#38;_version=1&#38;_urlVersion=0&#38;_userid=1029198&#38;md5=407fdea04f356c3be857377216278a44}, citeulike-linkout-1 = {http://dx.doi.org/10.1016/j.bbrc.2007.04.092}, doi = {10.1016/j.bbrc.2007.04.092}, journal = {Biochem Biophys Res Commun}, keywords = {2, acid, acids, b, ccl2, cell, chemokine, chemokines, chemotaxis, cxc, cxcl10, cxcl9, cyclooxygenase, expression, fatty, file-import-08-07-27, gene, humans, interleukin-8, leukocyte, line, macrophages, membrane, nf-kappa, nonesterified, palmitic, proteins, regulation}, local-url = {file://localhost/Users/ken/Documents/Papers/2007/Laine/Biochem\%20Biophys\%20Res\%20Commun\%202007\%20Laine.pdf}, month = {Jun}, number = {1}, pages = {150--5}, posted-at = {2008-07-27 14:46:02}, priority = {2}, title = {Palmitic acid induces IP-10 expression in human macrophages via NF-kappaB activation}, url = {http://dx.doi.org/10.1016/j.bbrc.2007.04.092}, volume = {358}, year = {2007} }

TY - JOUR ID - Laine:2007p722 L3 - citeulike-article-id:3046185 N2 - It is now recognized that cross-talk between adipocytes and adipose tissue stromal cells such as macrophages contributes to local and systemic inflammation. One factor from adipocytes that may participate in this interaction and that is frequently elevated in inflammatory conditions such as obesity, insulin resistance, and type 2 diabetes is free fatty acids (FFA). To investigate the potential for FFA to enhance macrophage inflammation, we exposed U937 macrophages to physiological levels (150 microM) of FFA. Palmitic acid (PA), the predominant saturated FFA released from adipose tissue, but not unsaturated FFA, induced an approximately 6-fold (p<0.05) increase in IP-10 gene expression (and 2- to 4-fold increases in IL-8, MCP-1, COX-2, and MIG). PA also induced an approximately 2-fold increase (p<0.05) in active NF-kappaB, and two structurally distinct NF-kappaB inhibitors effectively blocked PA-induced IP-10 gene expression. Conditioned medium from PA-treated cells increased lymphocyte migration 41% (p<0.05) which was significantly reduced by IP-10-neutralizing antibody. These results suggest that elevated concentrations of PA commonly present in obese and insulin resistant individuals can increase NF-kappaB-mediated expression of IP-10 in macrophages. These events in turn may lead to an increasing feed-forward loop of chronic inflammation. IS - 1 JF - Biochem Biophys Res Commun EP - 5 TI - Palmitic acid induces IP-10 expression in human macrophages via NF-kappaB activation VL - 358 SP - 150 KW - 2 KW - acid KW - acids KW - b KW - ccl2 KW - cell KW - chemokine KW - chemokines KW - chemotaxis KW - cxc KW - cxcl10 KW - cxcl9 KW - cyclooxygenase KW - expression KW - fatty KW - file-import-08-07-27 KW - gene KW - humans KW - interleukin-8 KW - leukocyte KW - line KW - macrophages KW - membrane KW - nf-kappa KW - nonesterified KW - palmitic KW - proteins KW - regulation AU - Laine, Phyllis AU - Schwartz, Eric AU - Wang, Yingjie AU - Zhang, Wei AU - Karnik, Sheetal AU - Musi, Nicolas AU - Reaven, Peter PY - 2007/Jun// UR - http://dx.doi.org/10.1016/j.bbrc.2007.04.092 ER -