Direct monitoring of in vivo ER stress during the development of insulin resistance with ER stress-activated indicator transgenic mice Export

@article{citeulike:3287412, abstract = {Type 2 diabetes is one of the most prevalent and serious metabolic diseases in the world, and insulin resistance and pancreatic [beta]-cell dysfunction are the hallmarks of the disease. It has been suggested that endoplasmic reticulum (ER) stress is provoked under diabetic conditions and is possibly involved in the development of insulin resistance. In this study, using ER stress-activated indicator (ERAI) transgenic mice which express green fluorescent protein (GFP) under ER stress conditions, we directly monitored in vivo ER stress in various insulin target tissues such as liver, fat, and muscle in diabetic mice with insulin resistance induced by high fat and high sucrose (HF/HS) diet treatment. In the liver of the ERAI transgenic mice, ERAI fluorescence activity was clearly observed as early as after 4 weeks of HF/HS diet treatment, whereas it was not detected at all in the fat and muscle even after 12 weeks of HF/HS diet treatment. These results suggest that induction of ER stress is associated with the development of insulin resistance and that ER stress in the liver may facilitate the development of insulin resistance in the whole body. This is the first report to directly monitor in vivo ER stress in various insulin target tissues during the development of insulin resistance. In addition, our present results suggest that ERAI transgenic mice are very useful for evaluating in vivo ER stress, especially in the liver, during the development of insulin resistance.}, author = {Yoshiuchi, Kazutomi and Kaneto, Hideaki and Matsuoka, Taka-Aki and Kohno, Kenji and Iwawaki, Takao and Nakatani, Yoshihisa and Yamasaki, Yoshimitsu and Hori, Masatsugu and Matsuhisa, Munehide}, citeulike-article-id = {3287412}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.bbrc.2007.11.182}, citeulike-linkout-1 = {http://www.sciencedirect.com/science/article/B6WBK-4R9YFK8-8/2/8bc12842ac23033126516e7f7f86f318}, day = {8}, doi = {10.1016/j.bbrc.2007.11.182}, journal = {Biochemical and Biophysical Research Communications}, month = {February}, number = {2}, pages = {545--550}, posted-at = {2008-09-18 15:16:57}, priority = {5}, title = {Direct monitoring of in vivo ER stress during the development of insulin resistance with ER stress-activated indicator transgenic mice}, url = {http://dx.doi.org/10.1016/j.bbrc.2007.11.182}, volume = {366}, year = {2008} }

TY - JOUR ID - citeulike:3287412 L3 - citeulike-article-id:3287412 N2 - Type 2 diabetes is one of the most prevalent and serious metabolic diseases in the world, and insulin resistance and pancreatic [beta]-cell dysfunction are the hallmarks of the disease. It has been suggested that endoplasmic reticulum (ER) stress is provoked under diabetic conditions and is possibly involved in the development of insulin resistance. In this study, using ER stress-activated indicator (ERAI) transgenic mice which express green fluorescent protein (GFP) under ER stress conditions, we directly monitored in vivo ER stress in various insulin target tissues such as liver, fat, and muscle in diabetic mice with insulin resistance induced by high fat and high sucrose (HF/HS) diet treatment. In the liver of the ERAI transgenic mice, ERAI fluorescence activity was clearly observed as early as after 4 weeks of HF/HS diet treatment, whereas it was not detected at all in the fat and muscle even after 12 weeks of HF/HS diet treatment. These results suggest that induction of ER stress is associated with the development of insulin resistance and that ER stress in the liver may facilitate the development of insulin resistance in the whole body. This is the first report to directly monitor in vivo ER stress in various insulin target tissues during the development of insulin resistance. In addition, our present results suggest that ERAI transgenic mice are very useful for evaluating in vivo ER stress, especially in the liver, during the development of insulin resistance. IS - 2 JF - Biochemical and Biophysical Research Communications EP - 550 TI - Direct monitoring of in vivo ER stress during the development of insulin resistance with ER stress-activated indicator transgenic mice VL - 366 SP - 545 AU - Yoshiuchi, Kazutomi AU - Kaneto, Hideaki AU - Matsuoka, Taka-Aki AU - Kohno, Kenji AU - Iwawaki, Takao AU - Nakatani, Yoshihisa AU - Yamasaki, Yoshimitsu AU - Hori, Masatsugu AU - Matsuhisa, Munehide PY - 2008/02/08/ UR - http://dx.doi.org/10.1016/j.bbrc.2007.11.182 ER -