Control of yeast mating signal transduction by a mammalian beta 2-adrenergic receptor and Gs alpha subunit. Export

@article{citeulike:3870846, abstract = {To facilitate functional and mechanistic studies of receptor-G protein interactions, [corrected] the human beta 2-adrenergic receptor (h beta-AR) has been expressed in Saccharomyces cerevisiae. This was achieved by placing a modified h beta-AR gene under control of the galactose-inducible GAL1 promoter. After induction by galactose, functional h beta-AR was expressed at a concentration several hundred times as great as that found in any human tissue. As determined from competitive ligand binding experiments, h beta-AR expressed in yeast displayed characteristic affinities, specificity, and stereoselectivity. Partial activation of the yeast pheromone response pathway by beta-adrenergic receptor agonists was achieved in cells coexpressing h beta-AR and a mammalian G protein (Gs) alpha subunit-demonstrating that these components can couple to each other and to downstream effectors when expressed in yeast. This in vivo reconstitution system provides a new approach for examining ligand binding and G protein coupling to cell surface receptors.}, author = {King, K. and Dohlman, H. G. and Thorner, J. and Caron, M. G. and Lefkowitz, R. J.}, citeulike-article-id = {3870846}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/2171146}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=2171146}, day = {5}, issn = {0036-8075}, journal = {Science (New York, N.Y.)}, keywords = {beta-adrenergic, receptor, yeast}, month = {October}, number = {4977}, pages = {121--123}, posted-at = {2009-01-09 09:34:28}, priority = {1}, title = {Control of yeast mating signal transduction by a mammalian beta 2-adrenergic receptor and Gs alpha subunit.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/2171146}, volume = {250}, year = {1990} }

TY - JOUR ID - citeulike:3870846 L3 - citeulike-article-id:3870846 N2 - To facilitate functional and mechanistic studies of receptor-G protein interactions, [corrected] the human beta 2-adrenergic receptor (h beta-AR) has been expressed in Saccharomyces cerevisiae. This was achieved by placing a modified h beta-AR gene under control of the galactose-inducible GAL1 promoter. After induction by galactose, functional h beta-AR was expressed at a concentration several hundred times as great as that found in any human tissue. As determined from competitive ligand binding experiments, h beta-AR expressed in yeast displayed characteristic affinities, specificity, and stereoselectivity. Partial activation of the yeast pheromone response pathway by beta-adrenergic receptor agonists was achieved in cells coexpressing h beta-AR and a mammalian G protein (Gs) alpha subunit-demonstrating that these components can couple to each other and to downstream effectors when expressed in yeast. This in vivo reconstitution system provides a new approach for examining ligand binding and G protein coupling to cell surface receptors. IS - 4977 JF - Science (New York, N.Y.) SN - 0036-8075 EP - 123 TI - Control of yeast mating signal transduction by a mammalian beta 2-adrenergic receptor and Gs alpha subunit. VL - 250 SP - 121 KW - beta-adrenergic KW - receptor KW - yeast AU - King, K AU - Dohlman, HG AU - Thorner, J AU - Caron, MG AU - Lefkowitz, RJ PY - 1990/10/05/ UR - http://view.ncbi.nlm.nih.gov/pubmed/2171146 ER -