Yeast assays for G-protein-coupled receptors. Export

@article{citeulike:3870856, abstract = {Yeast assays for G-protein-coupled receptors have many attractions due to their simplicity, low cost, and lack of endogenous receptors. Since the first report of functional coupling of the human beta 2 adrenergic receptor to the yeast pheromone-response pathway in 1990, the technology has developed to a point at which more than 30 heterologous GPCRs are now published to couple. Major breakthroughs have come from an understanding of receptor-G protein interactions, alongside advances in knowledge of the structure of heterotrimeric G proteins. Yeast screens have been used to identify ligands both from compound collections and through the autocrine expression of peptide libraries. Yeast genetics has also been applied to a functional analysis of GPCRs and peptide ligands. In this review we describe the historical development of yeast GPCR assay systems and their current applications.}, author = {Dowell, S. J. and Brown, A. J.}, citeulike-article-id = {3870856}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/12690961}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=12690961}, issn = {1060-6823}, journal = {Receptors \& channels}, keywords = {beta-adrenergic, g, gpcr, protein, yeast}, number = {5-6}, pages = {343--352}, posted-at = {2009-01-09 09:44:00}, priority = {2}, title = {Yeast assays for G-protein-coupled receptors.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/12690961}, volume = {8}, year = {2002} }

TY - JOUR ID - citeulike:3870856 L3 - citeulike-article-id:3870856 N2 - Yeast assays for G-protein-coupled receptors have many attractions due to their simplicity, low cost, and lack of endogenous receptors. Since the first report of functional coupling of the human beta 2 adrenergic receptor to the yeast pheromone-response pathway in 1990, the technology has developed to a point at which more than 30 heterologous GPCRs are now published to couple. Major breakthroughs have come from an understanding of receptor-G protein interactions, alongside advances in knowledge of the structure of heterotrimeric G proteins. Yeast screens have been used to identify ligands both from compound collections and through the autocrine expression of peptide libraries. Yeast genetics has also been applied to a functional analysis of GPCRs and peptide ligands. In this review we describe the historical development of yeast GPCR assay systems and their current applications. IS - 5-6 JF - Receptors & channels SN - 1060-6823 EP - 352 TI - Yeast assays for G-protein-coupled receptors. VL - 8 SP - 343 KW - beta-adrenergic KW - g KW - gpcr KW - protein KW - yeast AU - Dowell, SJ AU - Brown, AJ PY - 2002/// UR - http://view.ncbi.nlm.nih.gov/pubmed/12690961 ER -