High-dimensional and large-scale phenotyping of yeast mutants. Export

@article{citeulike:553833, abstract = {One of the most powerful techniques for attributing functions to genes in uni- and multicellular organisms is comprehensive analysis of mutant traits. In this study, systematic and quantitative analyses of mutant traits are achieved in the budding yeast Saccharomyces cerevisiae by investigating morphological phenotypes. Analysis of fluorescent microscopic images of triple-stained cells makes it possible to treat morphological variations as quantitative traits. Deletion of nearly half of the yeast genes not essential for growth affects these morphological traits. Similar morphological phenotypes are caused by deletions of functionally related genes, enabling a functional assignment of a locus to a specific cellular pathway. The high-dimensional phenotypic analysis of defined yeast mutant strains provides another step toward attributing gene function to all of the genes in the yeast genome.}, author = {Ohya, Yoshikazu and Sese, Jun and Yukawa, Masashi and Sano, Fumi and Nakatani, Yoichiro and Saito, Taro L. and Saka, Ayaka and Fukuda, Tomoyuki and Ishihara, Satoru and Oka, Satomi and Suzuki, Genjiro and Watanabe, Machika and Hirata, Aiko and Ohtani, Miwaka and Sawai, Hiroshi and Fraysse, Nicolas and Latg\'{e}, Jean-Paul P. and Fran\c{c}ois, Jean M. and Aebi, Markus and Tanaka, Seiji and Muramatsu, Sachiko and Araki, Hiroyuki and Sonoike, Kintake and Nogami, Satoru and Morishita, Shinichi}, citeulike-article-id = {553833}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0509436102}, citeulike-linkout-1 = {http://www.pnas.org/content/102/52/19015.abstract}, citeulike-linkout-2 = {http://www.pnas.org/content/102/52/19015.full.pdf}, citeulike-linkout-3 = {http://www.pnas.org/cgi/content/abstract/102/52/19015}, citeulike-linkout-4 = {http://view.ncbi.nlm.nih.gov/pubmed/16365294}, citeulike-linkout-5 = {http://www.hubmed.org/display.cgi?uids=16365294}, day = {27}, doi = {10.1073/pnas.0509436102}, issn = {0027-8424}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, keywords = {discovery, drug, in, vivo, yeast}, month = {December}, number = {52}, pages = {19015--19020}, posted-at = {2006-12-22 10:03:02}, priority = {5}, title = {High-dimensional and large-scale phenotyping of yeast mutants.}, url = {http://dx.doi.org/10.1073/pnas.0509436102}, volume = {102}, year = {2005} }

TY - JOUR ID - citeulike:553833 L3 - citeulike-article-id:553833 N2 - One of the most powerful techniques for attributing functions to genes in uni- and multicellular organisms is comprehensive analysis of mutant traits. In this study, systematic and quantitative analyses of mutant traits are achieved in the budding yeast Saccharomyces cerevisiae by investigating morphological phenotypes. Analysis of fluorescent microscopic images of triple-stained cells makes it possible to treat morphological variations as quantitative traits. Deletion of nearly half of the yeast genes not essential for growth affects these morphological traits. Similar morphological phenotypes are caused by deletions of functionally related genes, enabling a functional assignment of a locus to a specific cellular pathway. The high-dimensional phenotypic analysis of defined yeast mutant strains provides another step toward attributing gene function to all of the genes in the yeast genome. IS - 52 JF - Proceedings of the National Academy of Sciences of the United States of America SN - 0027-8424 EP - 19020 TI - High-dimensional and large-scale phenotyping of yeast mutants. VL - 102 SP - 19015 KW - discovery KW - drug KW - in KW - vivo KW - yeast AU - Ohya, Yoshikazu AU - Sese, Jun AU - Yukawa, Masashi AU - Sano, Fumi AU - Nakatani, Yoichiro AU - Saito, Taro AU - Saka, Ayaka AU - Fukuda, Tomoyuki AU - Ishihara, Satoru AU - Oka, Satomi AU - Suzuki, Genjiro AU - Watanabe, Machika AU - Hirata, Aiko AU - Ohtani, Miwaka AU - Sawai, Hiroshi AU - Fraysse, Nicolas AU - Latgé, Jean-Paul AU - François, Jean AU - Aebi, Markus AU - Tanaka, Seiji AU - Muramatsu, Sachiko AU - Araki, Hiroyuki AU - Sonoike, Kintake AU - Nogami, Satoru AU - Morishita, Shinichi PY - 2005/12/27/ UR - http://dx.doi.org/10.1073/pnas.0509436102 ER -