Regulation of human hepatocyte gene expression by fatty acids. Export

@article{citeulike:2190145, abstract = {It is known that fatty acids (FAs) regulate transcription through a number of FA responsive transcription factors. In order to investigate the effect of FAs on gene regulation in cultured human hepatocytes we examined the effect of palmitate on hepatic glucokinase (GK) promoter activity and expression of transcription factors that regulate GK expression. GK promoter activity was increased in constructs lacking a cAMP response element (CRE), while palmitate incubation decreased GK promoter activity in CRE-negative constructs. Cells exposed to palmitate showed increased levels of PPARalpha apolipoprotein-AII and -B100 mRNA and decreased levels of SREBP-1c mRNA but there was no effect on LXRalpha and HNF-4alpha mRNA. In addition, cDNA microarray analysis of short-term (1.5h) transcriptional regulation by palmitate, oleate, and eicosapentaenoic acid (EPA) identified that oleate and EPA initiated similar changes in the pattern of hepatic gene regulation, whereas palmitate had little effect.}, address = {Cooperative Research Centre for Diagnostics, Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Qld 4059, Australia.}, author = {Swagell, C. D. and Henly, D. C. and Morris, C. P.}, citeulike-article-id = {2190145}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.bbrc.2007.07.191}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/17707340}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=17707340}, day = {19}, doi = {10.1016/j.bbrc.2007.07.191}, issn = {0006-291X}, journal = {Biochem Biophys Res Commun}, keywords = {fattyacid, hnf4, ligands}, month = {October}, number = {2}, pages = {374--380}, posted-at = {2008-01-02 23:42:40}, priority = {2}, title = {Regulation of human hepatocyte gene expression by fatty acids.}, url = {http://dx.doi.org/10.1016/j.bbrc.2007.07.191}, volume = {362}, year = {2007} }

TY - JOUR ID - citeulike:2190145 L3 - citeulike-article-id:2190145 N2 - It is known that fatty acids (FAs) regulate transcription through a number of FA responsive transcription factors. In order to investigate the effect of FAs on gene regulation in cultured human hepatocytes we examined the effect of palmitate on hepatic glucokinase (GK) promoter activity and expression of transcription factors that regulate GK expression. GK promoter activity was increased in constructs lacking a cAMP response element (CRE), while palmitate incubation decreased GK promoter activity in CRE-negative constructs. Cells exposed to palmitate showed increased levels of PPARalpha apolipoprotein-AII and -B100 mRNA and decreased levels of SREBP-1c mRNA but there was no effect on LXRalpha and HNF-4alpha mRNA. In addition, cDNA microarray analysis of short-term (1.5h) transcriptional regulation by palmitate, oleate, and eicosapentaenoic acid (EPA) identified that oleate and EPA initiated similar changes in the pattern of hepatic gene regulation, whereas palmitate had little effect. IS - 2 JF - Biochem Biophys Res Commun SN - 0006-291X AD - Cooperative Research Centre for Diagnostics, Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Qld 4059, Australia. EP - 380 TI - Regulation of human hepatocyte gene expression by fatty acids. VL - 362 SP - 374 KW - fattyacid KW - hnf4 KW - ligands AU - Swagell, CD AU - Henly, DC AU - Morris, CP PY - 2007/10/19/ UR - http://dx.doi.org/10.1016/j.bbrc.2007.07.191 ER -