Cyanine dye dUTP analogs for enzymatic labeling of DNA probes. Export

@article{citeulike:2195708, abstract = {Fluorescence in situ hybridization (FISH) has become and indispensable tool in a variety of areas of research and clinical diagnostics. Many applications demand an approach for simultaneous detection of multiple target sequences that is rapid and simple, yet sensitive. In this work, we describe the synthesis of two new cyanine dye-labeled dUTP analogs, Cy3-dUTP and Cy5-dUTP. They are efficient substrates for DNA polymerases and can be incorporated into DNA probes by standard nick translation, random priming and polymerase chain reactions. Optimal labeling conditions have been identified which yield probes with 20-40 dyes per kilobase. The directly labeled DNA probes obtained with these analogs offer a simple approach for multicolor multisequence analysis that requires no secondary detection reagents and steps.}, address = {Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213.}, author = {Yu, H. and Chao, J. and Patek, D. and Mujumdar, R. and Mujumdar, S. and Waggoner, A. S.}, citeulike-article-id = {2195708}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/8065939}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=8065939}, day = {11}, issn = {0305-1048}, journal = {Nucleic Acids Res}, keywords = {dutp, labeling, microarray}, month = {August}, number = {15}, pages = {3226--3232}, posted-at = {2008-01-04 22:04:27}, priority = {2}, title = {Cyanine dye dUTP analogs for enzymatic labeling of DNA probes.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/8065939}, volume = {22}, year = {1994} }

TY - JOUR ID - citeulike:2195708 L3 - citeulike-article-id:2195708 N2 - Fluorescence in situ hybridization (FISH) has become and indispensable tool in a variety of areas of research and clinical diagnostics. Many applications demand an approach for simultaneous detection of multiple target sequences that is rapid and simple, yet sensitive. In this work, we describe the synthesis of two new cyanine dye-labeled dUTP analogs, Cy3-dUTP and Cy5-dUTP. They are efficient substrates for DNA polymerases and can be incorporated into DNA probes by standard nick translation, random priming and polymerase chain reactions. Optimal labeling conditions have been identified which yield probes with 20-40 dyes per kilobase. The directly labeled DNA probes obtained with these analogs offer a simple approach for multicolor multisequence analysis that requires no secondary detection reagents and steps. IS - 15 JF - Nucleic Acids Res SN - 0305-1048 AD - Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213. EP - 3232 TI - Cyanine dye dUTP analogs for enzymatic labeling of DNA probes. VL - 22 SP - 3226 KW - dutp KW - labeling KW - microarray AU - Yu, H AU - Chao, J AU - Patek, D AU - Mujumdar, R AU - Mujumdar, S AU - Waggoner, AS PY - 1994/08/11/ UR - http://view.ncbi.nlm.nih.gov/pubmed/8065939 ER -