Cyanine dye labeling reagents: sulfoindocyanine succinimidyl esters. Export

@article{citeulike:2195710, abstract = {A series of new fluorescent labeling reagents based on sulfoindocyanine dyes has been developed. We describe the synthesis and properties of these reagents. They contain succinimidyl ester reactive groups and can be readily conjugated to antibodies, avidin, DNA, lipids, polymers, and other amino-group-containing materials. The labeling reagents are water soluble, pH insensitive, and show much reduced dye aggregation under labeling conditions. One of the reagents, Cy3, can be excited with the 488-, 514- and 532-nm laser lines and is optimally excited with the 546-nm mercury arc line. Another, Cy5, can be excited with the 633-nm HeNe and 647-nm Kr laser lines available with many flow cytometers and confocal laser-scanning microscopes. New laser diodes emitting near 650 nm should also be excellent excitation sources for Cy5.}, address = {Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213.}, author = {Mujumdar, R. B. and Ernst, L. A. and Mujumdar, S. R. and Lewis, C. J. and Waggoner, A. S.}, citeulike-article-id = {2195710}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/7873641}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=7873641}, issn = {1043-1802}, journal = {Bioconjug Chem}, keywords = {dutp, labeling}, number = {2}, pages = {105--111}, posted-at = {2008-01-04 22:04:59}, priority = {2}, title = {Cyanine dye labeling reagents: sulfoindocyanine succinimidyl esters.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/7873641}, volume = {4}, year = {1993} }

TY - JOUR ID - citeulike:2195710 L3 - citeulike-article-id:2195710 N2 - A series of new fluorescent labeling reagents based on sulfoindocyanine dyes has been developed. We describe the synthesis and properties of these reagents. They contain succinimidyl ester reactive groups and can be readily conjugated to antibodies, avidin, DNA, lipids, polymers, and other amino-group-containing materials. The labeling reagents are water soluble, pH insensitive, and show much reduced dye aggregation under labeling conditions. One of the reagents, Cy3, can be excited with the 488-, 514- and 532-nm laser lines and is optimally excited with the 546-nm mercury arc line. Another, Cy5, can be excited with the 633-nm HeNe and 647-nm Kr laser lines available with many flow cytometers and confocal laser-scanning microscopes. New laser diodes emitting near 650 nm should also be excellent excitation sources for Cy5. IS - 2 JF - Bioconjug Chem SN - 1043-1802 AD - Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213. EP - 111 TI - Cyanine dye labeling reagents: sulfoindocyanine succinimidyl esters. VL - 4 SP - 105 KW - dutp KW - labeling AU - Mujumdar, RB AU - Ernst, LA AU - Mujumdar, SR AU - Lewis, CJ AU - Waggoner, AS PY - 1993///r UR - http://view.ncbi.nlm.nih.gov/pubmed/7873641 ER -