Genetic engineering of human embryonic stem cells with lentiviral vectors. Export

@article{citeulike:2204757, abstract = {Human embryonic stem (hES) cells present a valuable source of cells with a vast therapeutic potential. However, the low efficiency of directed differentiation of hES cells remains a major obstacle in their uses for regenerative medicine. While differentiation may be controlled by the genetic manipulation, effective and efficient gene transfer into hES cells has been an elusive goal. Here, we show stable and efficient genetic manipulations of hES cells using lentiviral vectors. This method resulted in the establishment of stable gene expression without loss of pluripotency in hES cells. In addition, lentiviral vectors were effective in conveying the expression of an U6 promoter-driven small interfering RNA (siRNA), which was effective in silencing its specific target. Taken together, our results suggest that lentiviral gene delivery holds great promise for hES cell research and application.}, address = {Cardiovascular Research Institute, Morehouse School of Medicine, Atlanta, GA 30310, USA.}, author = {Xiong, C. and Tang, D. Q. and Xie, C. Q. and Zhang, L. and Xu, K. F. and Thompson, W. E. and Chou, W. and Gibbons, G. H. and Chang, L. J. and Yang, L. J. and Chen, Y. E.}, citeulike-article-id = {2204757}, citeulike-linkout-0 = {http://dx.doi.org/10.1089/scd.2005.14.367}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/16137225}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=16137225}, doi = {10.1089/scd.2005.14.367}, issn = {1547-3287}, journal = {Stem Cells Dev}, keywords = {cells, lentivirus, stem}, month = {August}, number = {4}, pages = {367--377}, posted-at = {2008-01-07 19:52:14}, priority = {1}, title = {Genetic engineering of human embryonic stem cells with lentiviral vectors.}, url = {http://dx.doi.org/10.1089/scd.2005.14.367}, volume = {14}, year = {2005} }

TY - JOUR ID - citeulike:2204757 L3 - citeulike-article-id:2204757 N2 - Human embryonic stem (hES) cells present a valuable source of cells with a vast therapeutic potential. However, the low efficiency of directed differentiation of hES cells remains a major obstacle in their uses for regenerative medicine. While differentiation may be controlled by the genetic manipulation, effective and efficient gene transfer into hES cells has been an elusive goal. Here, we show stable and efficient genetic manipulations of hES cells using lentiviral vectors. This method resulted in the establishment of stable gene expression without loss of pluripotency in hES cells. In addition, lentiviral vectors were effective in conveying the expression of an U6 promoter-driven small interfering RNA (siRNA), which was effective in silencing its specific target. Taken together, our results suggest that lentiviral gene delivery holds great promise for hES cell research and application. IS - 4 JF - Stem Cells Dev SN - 1547-3287 AD - Cardiovascular Research Institute, Morehouse School of Medicine, Atlanta, GA 30310, USA. EP - 377 TI - Genetic engineering of human embryonic stem cells with lentiviral vectors. VL - 14 SP - 367 KW - cells KW - lentivirus KW - stem AU - Xiong, C AU - Tang, DQ AU - Xie, CQ AU - Zhang, L AU - Xu, KF AU - Thompson, WE AU - Chou, W AU - Gibbons, GH AU - Chang, LJ AU - Yang, LJ AU - Chen, YE PY - 2005/08// UR - http://dx.doi.org/10.1089/scd.2005.14.367 ER -