Characterization of a novel EGFP reporter mouse to monitor Cre recombination as demonstrated by a Tie2 Cre mouse line Export

@article{citeulike:2973267, abstract = {Summary: The use of the Cre/loxP system has greatly empowered the field of gene targeting. Here we describe the successful establishment of a novel knock-in EGFP reporter mouse line to monitor Cre-induced recombination in the vast majority of cell types. The value of this reporter mouse line is demonstrated by the use of a novel Tie2Cre transgenic mouse line that facilitates gene targeting in endothelial and hematopoietic cells. High efficiency of recombination was found in all endothelial cells and in the majority of hematopoietic cells but was absent in other tissues. Furthermore, in the second generation, the Tie2Cre mouse can be used to get 100\% recombination of one allele, whilst allowing tissue specific in the second, therefore offering excellent efficiency. genesis 30:36-44, 2001. {\copyright} 2001 Wiley-Liss, Inc.}, address = {Department of Molecular Immunology, Division of Tumor Immunology, German Cancer Research Center, Heidelberg, Germany; Department of Cellular and Molecular Pathology, German Cancer Research Center, Heidelberg, Germany; Department of Internal Medicine I, University of Heidelberg, Heidelberg, Germany}, author = {Constien, Rainer and Forde, Anne and Liliensiek, Birgit and Gr\"{o}ne, Hermann-Josef and Nawroth, Peter and H\"{a}mmerling, G\"{u}nter and Arnold, Bernd}, citeulike-article-id = {2973267}, citeulike-linkout-0 = {http://dx.doi.org/10.1002/gene.1030}, citeulike-linkout-1 = {http://www3.interscience.wiley.com/cgi-bin/abstract/81002371/ABSTRACT}, doi = {10.1002/gene.1030}, journal = {genesis}, keywords = {loxp, mice, rage}, number = {1}, pages = {36--44}, posted-at = {2008-07-08 20:40:24}, priority = {2}, title = {Characterization of a novel EGFP reporter mouse to monitor Cre recombination as demonstrated by a Tie2 Cre mouse line}, url = {http://dx.doi.org/10.1002/gene.1030}, volume = {30}, year = {2001} }

TY - JOUR ID - citeulike:2973267 L3 - citeulike-article-id:2973267 N2 - Summary: The use of the Cre/loxP system has greatly empowered the field of gene targeting. Here we describe the successful establishment of a novel knock-in EGFP reporter mouse line to monitor Cre-induced recombination in the vast majority of cell types. The value of this reporter mouse line is demonstrated by the use of a novel Tie2Cre transgenic mouse line that facilitates gene targeting in endothelial and hematopoietic cells. High efficiency of recombination was found in all endothelial cells and in the majority of hematopoietic cells but was absent in other tissues. Furthermore, in the second generation, the Tie2Cre mouse can be used to get 100% recombination of one allele, whilst allowing tissue specific in the second, therefore offering excellent efficiency. genesis 30:36-44, 2001. © 2001 Wiley-Liss, Inc. IS - 1 JF - genesis AD - Department of Molecular Immunology, Division of Tumor Immunology, German Cancer Research Center, Heidelberg, Germany; Department of Cellular and Molecular Pathology, German Cancer Research Center, Heidelberg, Germany; Department of Internal Medicine I, University of Heidelberg, Heidelberg, Germany EP - 44 TI - Characterization of a novel EGFP reporter mouse to monitor Cre recombination as demonstrated by a Tie2 Cre mouse line VL - 30 SP - 36 KW - loxp KW - mice KW - rage AU - Constien, Rainer AU - Forde, Anne AU - Liliensiek, Birgit AU - Gröne, Hermann-Josef AU - Nawroth, Peter AU - Hämmerling, Günter AU - Arnold, Bernd PY - 2001/// UR - http://dx.doi.org/10.1002/gene.1030 ER -