A Na+- and Cl--activated K+ Channel in the Thick Ascending Limb of Mouse Kidney Export

@article{citeulike:486655, abstract = {This study investigates the presence and properties of Na+-activated K+ (KNa) channels in epithelial renal cells. Using real-time PCR on mouse microdissected nephron segments, we show that Slo2.2 mRNA, which encodes for the KNa channels of excitable cells, is expressed in the medullary and cortical thick ascending limbs of Henle's loop, but not in the other parts of the nephron. Patch-clamp analysis revealed the presence of a high conductance K+ channel in the basolateral membrane of both the medullary and cortical thick ascending limbs. This channel was highly K+ selective (PK/PNa [\~{}] 20), its conductance ranged from 140 to 180 pS with subconductance levels, and its current/voltage relationship displayed intermediate, Na+-dependent, inward rectification. Internal Na+ and Cl- activated the channel with 50\% effective concentrations (EC50) and Hill coefficients (nH) of 30 {+/-} 1 mM and 3.9 {+/-} 0.5 for internal Na+, and 35 {+/-} 10 mM and 1.3 {+/-} 0.25 for internal Cl-. Channel activity was unaltered by internal ATP (2 mM) and by internal pH, but clearly decreased when internal free Ca2+ concentration increased. This is the first demonstration of the presence in the epithelial cell membrane of a functional, Na+-activated, large-conductance K+ channel that closely resembles native KNa channels of excitable cells. This Slo2.2 type, Na+- and Cl--activated K+ channel is primarily located in the thick ascending limb, a major renal site of transcellular NaCl reabsorption.}, author = {Paulais, Marc and Lachheb, Sahran and Teulon, Jacques}, citeulike-article-id = {486655}, citeulike-linkout-0 = {http://dx.doi.org/10.1085/jgp.200509360}, citeulike-linkout-1 = {http://www.jgp.org/cgi/content/abstract/127/2/205}, day = {30}, doi = {10.1085/jgp.200509360}, journal = {J. Gen. Physiol.}, keywords = {channel, kidney, kna, tal}, month = {January}, number = {2}, pages = {205--215}, posted-at = {2006-01-31 02:07:52}, priority = {4}, title = {A Na+- and Cl--activated K+ Channel in the Thick Ascending Limb of Mouse Kidney}, url = {http://dx.doi.org/10.1085/jgp.200509360}, volume = {127}, year = {2006} }

TY - JOUR ID - citeulike:486655 L3 - citeulike-article-id:486655 N2 - This study investigates the presence and properties of Na+-activated K+ (KNa) channels in epithelial renal cells. Using real-time PCR on mouse microdissected nephron segments, we show that Slo2.2 mRNA, which encodes for the KNa channels of excitable cells, is expressed in the medullary and cortical thick ascending limbs of Henle's loop, but not in the other parts of the nephron. Patch-clamp analysis revealed the presence of a high conductance K+ channel in the basolateral membrane of both the medullary and cortical thick ascending limbs. This channel was highly K+ selective (PK/PNa [~] 20), its conductance ranged from 140 to 180 pS with subconductance levels, and its current/voltage relationship displayed intermediate, Na+-dependent, inward rectification. Internal Na+ and Cl- activated the channel with 50% effective concentrations (EC50) and Hill coefficients (nH) of 30 {+/-} 1 mM and 3.9 {+/-} 0.5 for internal Na+, and 35 {+/-} 10 mM and 1.3 {+/-} 0.25 for internal Cl-. Channel activity was unaltered by internal ATP (2 mM) and by internal pH, but clearly decreased when internal free Ca2+ concentration increased. This is the first demonstration of the presence in the epithelial cell membrane of a functional, Na+-activated, large-conductance K+ channel that closely resembles native KNa channels of excitable cells. This Slo2.2 type, Na+- and Cl--activated K+ channel is primarily located in the thick ascending limb, a major renal site of transcellular NaCl reabsorption. IS - 2 JF - J. Gen. Physiol. EP - 215 TI - A Na+- and Cl--activated K+ Channel in the Thick Ascending Limb of Mouse Kidney VL - 127 SP - 205 KW - channel KW - kidney KW - kna KW - tal AU - Paulais, Marc AU - Lachheb, Sahran AU - Teulon, Jacques PY - 2006/01/30/ UR - http://dx.doi.org/10.1085/jgp.200509360 ER -