Neuromodulation of Na(+) Channel Slow Inactivation via cAMP-Dependent Protein Kinase and Protein Kinase C. Export

@article{citeulike:488971, abstract = {Neurotransmitters modulate sodium channel availability through activation of G protein-coupled receptors, cAMP-dependent protein kinase (PKA), and protein kinase C (PKC). Voltage-dependent slow inactivation also controls sodium channel availability, synaptic integration, and neuronal firing. Here we show by analysis of sodium channel mutants that neuromodulation via PKA and PKC enhances intrinsic slow inactivation of sodium channels, making them unavailable for activation. Mutations in the S6 segment in domain III (N1466A,D) either enhance or block slow inactivation, implicating S6 segments in the molecular pathway for slow inactivation. Modulation of N1466A channels by PKC or PKA is increased, whereas modulation of N1466D is nearly completely blocked. These results demonstrate that neuromodulation by PKA and PKC is caused by their enhancement of intrinsic slow inactivation gating. Modulation of slow inactivation by neurotransmitters acting through G protein-coupled receptors, PKA, and PKC is a flexible mechanism of cellular plasticity controlling the firing behavior of central neurons.}, address = {Department of Pharmacology, University of Washington, Seattle, Washington 98195.}, author = {Chen, Y. and Yu, F. H. and Surmeier, D. J. and Scheuer, T. and Catterall, W. A.}, citeulike-article-id = {488971}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.neuron.2006.01.009}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/16446144}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=16446144}, day = {2}, doi = {10.1016/j.neuron.2006.01.009}, issn = {0896-6273}, journal = {Neuron}, keywords = {channel, pka, pkc}, month = {February}, number = {3}, pages = {409--420}, posted-at = {2006-02-02 00:40:38}, priority = {2}, title = {Neuromodulation of Na(+) Channel Slow Inactivation via cAMP-Dependent Protein Kinase and Protein Kinase C.}, url = {http://dx.doi.org/10.1016/j.neuron.2006.01.009}, volume = {49}, year = {2006} }

TY - JOUR ID - citeulike:488971 L3 - citeulike-article-id:488971 N2 - Neurotransmitters modulate sodium channel availability through activation of G protein-coupled receptors, cAMP-dependent protein kinase (PKA), and protein kinase C (PKC). Voltage-dependent slow inactivation also controls sodium channel availability, synaptic integration, and neuronal firing. Here we show by analysis of sodium channel mutants that neuromodulation via PKA and PKC enhances intrinsic slow inactivation of sodium channels, making them unavailable for activation. Mutations in the S6 segment in domain III (N1466A,D) either enhance or block slow inactivation, implicating S6 segments in the molecular pathway for slow inactivation. Modulation of N1466A channels by PKC or PKA is increased, whereas modulation of N1466D is nearly completely blocked. These results demonstrate that neuromodulation by PKA and PKC is caused by their enhancement of intrinsic slow inactivation gating. Modulation of slow inactivation by neurotransmitters acting through G protein-coupled receptors, PKA, and PKC is a flexible mechanism of cellular plasticity controlling the firing behavior of central neurons. IS - 3 JF - Neuron SN - 0896-6273 AD - Department of Pharmacology, University of Washington, Seattle, Washington 98195. EP - 420 TI - Neuromodulation of Na(+) Channel Slow Inactivation via cAMP-Dependent Protein Kinase and Protein Kinase C. VL - 49 SP - 409 KW - channel KW - pka KW - pkc AU - Chen, Y AU - Yu, FH AU - Surmeier, DJ AU - Scheuer, T AU - Catterall, WA PY - 2006/02/02/ UR - http://dx.doi.org/10.1016/j.neuron.2006.01.009 ER -