lazaro Encodes a Lipid Phosphate Phosphohydrolase that Regulates Phosphatidylinositol Turnover during Drosophila Phototransduction. Export

@article{citeulike:507371, abstract = {An essential step in Drosophila phototransduction is the hydrolysis of phosphatidylinositol 4,5 bisphosphate PI(4,5)P(2) by phospholipase Cbeta (PLCbeta) to generate a second messenger that opens the light-activated channels TRP and TRPL. Although the identity of this messenger remains unknown, recent evidence has implicated diacylglycerol kinase (DGK), encoded by rdgA, as a key enzyme that regulates its levels, mediating both amplification and response termination. In this study, we demonstrate that lazaro (laza) encodes a lipid phosphate phosphohydrolase (LPP) that functions during phototransduction. We demonstrate that the synergistic activity of laza and rdgA regulates response termination during phototransduction. Analysis of retinal phospholipids revealed a reduction in phosphatidic acid (PA) levels and an associated reduction in phosphatidylinositol (PI) levels. Together our results demonstrate the contribution of PI depletion to the rdgA phenotype and provide evidence that depletion of PI and its metabolites might be a key signal for TRP channel activation in vivo.}, address = {Inositide Laboratory, Babraham Institute, Babraham Research Campus, Cambridge CB2 4AT, United Kingdom.}, author = {Garcia-Murillas, I. and Pettitt, T. and Macdonald, E. and Okkenhaug, H. and Georgiev, P. and Trivedi, D. and Hassan, B. and Wakelam, M. and Raghu, P.}, citeulike-article-id = {507371}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.neuron.2006.02.001}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/16476663}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=16476663}, day = {16}, doi = {10.1016/j.neuron.2006.02.001}, issn = {0896-6273}, journal = {Neuron}, keywords = {channel, drosophila, phototransduction, trp}, month = {February}, number = {4}, pages = {533--546}, posted-at = {2006-02-17 00:03:55}, priority = {3}, title = {lazaro Encodes a Lipid Phosphate Phosphohydrolase that Regulates Phosphatidylinositol Turnover during Drosophila Phototransduction.}, url = {http://dx.doi.org/10.1016/j.neuron.2006.02.001}, volume = {49}, year = {2006} }

TY - JOUR ID - citeulike:507371 L3 - citeulike-article-id:507371 N2 - An essential step in Drosophila phototransduction is the hydrolysis of phosphatidylinositol 4,5 bisphosphate PI(4,5)P(2) by phospholipase Cbeta (PLCbeta) to generate a second messenger that opens the light-activated channels TRP and TRPL. Although the identity of this messenger remains unknown, recent evidence has implicated diacylglycerol kinase (DGK), encoded by rdgA, as a key enzyme that regulates its levels, mediating both amplification and response termination. In this study, we demonstrate that lazaro (laza) encodes a lipid phosphate phosphohydrolase (LPP) that functions during phototransduction. We demonstrate that the synergistic activity of laza and rdgA regulates response termination during phototransduction. Analysis of retinal phospholipids revealed a reduction in phosphatidic acid (PA) levels and an associated reduction in phosphatidylinositol (PI) levels. Together our results demonstrate the contribution of PI depletion to the rdgA phenotype and provide evidence that depletion of PI and its metabolites might be a key signal for TRP channel activation in vivo. IS - 4 JF - Neuron SN - 0896-6273 AD - Inositide Laboratory, Babraham Institute, Babraham Research Campus, Cambridge CB2 4AT, United Kingdom. EP - 546 TI - lazaro Encodes a Lipid Phosphate Phosphohydrolase that Regulates Phosphatidylinositol Turnover during Drosophila Phototransduction. VL - 49 SP - 533 KW - channel KW - drosophila KW - phototransduction KW - trp AU - Garcia-Murillas, I AU - Pettitt, T AU - Macdonald, E AU - Okkenhaug, H AU - Georgiev, P AU - Trivedi, D AU - Hassan, B AU - Wakelam, M AU - Raghu, P PY - 2006/02/16/ UR - http://dx.doi.org/10.1016/j.neuron.2006.02.001 ER -