Flow cytometric characterization of T lymphocyte subsets in the peripheral blood of Chinese rhesus macaques: normal range, age- and sex-related differences. Export

@article{citeulike:3182988, abstract = {Available data on the normal levels of white blood cell populations in healthy rhesus macaques (Macaca mulatta) originated and living in China is scanty. To obtain such data, blood samples from 150 Chinese rhesus macaques were collected and the normal range of white blood cells and their subsets were analyzed according to age and sex by flow cytometry. CBC data showed that the count of total white blood cells and lymphocytes decreased with age. Phenotypic analysis of CD4 and CD8 expression on CD3+ T lymphocytes showed that the percentage of CD4+ T cells (51.4+/-9.6\%), CD4-CD8- T cells (8.5+/-4.1\%) and the ratio of CD4+ T to CD8+ T cells (1.26+/-0.55) decreased with age; and the percentage of CD8+ T cells (42.0+/-9.7\%), CD4+CD8+ T cells (1.3+/-0.9\%) and CD3+ lymphocytes (55.3+/-13.3\%) increased with age. However, no statistically significant difference was observed between the male and female groups in most parameters in these monkeys except for the percentage of CD4+CD8+ T cells. This study provided basic information about blood cell count and T lymphocyte subsets in Chinese rhesus macaques. It may be useful for comparative studies using Indian and Chinese rhesus macaques.}, address = {State Key Laboratory for Infectious Disease Control and Prevention, National Center for AIDS/STD Control and Prevention, China-CDC, 27 Nanwei Road, Xuanwu District, Beijing 100050, PR China.}, author = {Qiu, C. L. and Zhao, H. and Yang, G. B. and Liu, Q. and Shao, Y.}, citeulike-article-id = {3182988}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.vetimm.2008.04.005}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/18499268}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=18499268}, day = {15}, doi = {10.1016/j.vetimm.2008.04.005}, issn = {0165-2427}, journal = {Veterinary immunology and immunopathology}, keywords = {flow\_cytometry, lymphocyte, pbmc, rhesus}, month = {August}, number = {3-4}, pages = {313--321}, posted-at = {2008-09-02 21:46:07}, priority = {3}, title = {Flow cytometric characterization of T lymphocyte subsets in the peripheral blood of Chinese rhesus macaques: normal range, age- and sex-related differences.}, url = {http://dx.doi.org/10.1016/j.vetimm.2008.04.005}, volume = {124}, year = {2008} }

TY - JOUR ID - citeulike:3182988 L3 - citeulike-article-id:3182988 N2 - Available data on the normal levels of white blood cell populations in healthy rhesus macaques (Macaca mulatta) originated and living in China is scanty. To obtain such data, blood samples from 150 Chinese rhesus macaques were collected and the normal range of white blood cells and their subsets were analyzed according to age and sex by flow cytometry. CBC data showed that the count of total white blood cells and lymphocytes decreased with age. Phenotypic analysis of CD4 and CD8 expression on CD3+ T lymphocytes showed that the percentage of CD4+ T cells (51.4+/-9.6%), CD4-CD8- T cells (8.5+/-4.1%) and the ratio of CD4+ T to CD8+ T cells (1.26+/-0.55) decreased with age; and the percentage of CD8+ T cells (42.0+/-9.7%), CD4+CD8+ T cells (1.3+/-0.9%) and CD3+ lymphocytes (55.3+/-13.3%) increased with age. However, no statistically significant difference was observed between the male and female groups in most parameters in these monkeys except for the percentage of CD4+CD8+ T cells. This study provided basic information about blood cell count and T lymphocyte subsets in Chinese rhesus macaques. It may be useful for comparative studies using Indian and Chinese rhesus macaques. IS - 3-4 JF - Veterinary immunology and immunopathology SN - 0165-2427 AD - State Key Laboratory for Infectious Disease Control and Prevention, National Center for AIDS/STD Control and Prevention, China-CDC, 27 Nanwei Road, Xuanwu District, Beijing 100050, PR China. EP - 321 TI - Flow cytometric characterization of T lymphocyte subsets in the peripheral blood of Chinese rhesus macaques: normal range, age- and sex-related differences. VL - 124 SP - 313 KW - flow_cytometry KW - lymphocyte KW - pbmc KW - rhesus AU - Qiu, CL AU - Zhao, H AU - Yang, GB AU - Liu, Q AU - Shao, Y PY - 2008/08/15/ UR - http://dx.doi.org/10.1016/j.vetimm.2008.04.005 ER -