Peritoneal macrophages express the serotonin transporter. Export

@article{citeulike:3182989, abstract = {Although it is known that macrophages take up serotonin, a specific monoamine transporter has not been identified in macrophages. In this study, mRNA coding for the serotonin transporter (SERT) was detected with the reverse transcription-polymerase chain reaction (RT-PCR) in recruited mouse peritoneal macrophages. Sequencing confirmed the identity of the RT-PCR product to mouse SERT mRNA. SERT protein was detected by Western blotting. Macrophage activation with lipopolysaccharide had no effect on expression of SERT mRNA or protein. Consistent with expression of a functional SERT, specific uptake of (3)H-serotonin in macrophages was sodium dependent and inhibited by fluoxetine (IC(50) 6.9 nM) and desipramine (IC(50) 32 nM) but not by nisoxetine or reserpine.}, address = {Department of Biology, Virginia Commonwealth University, Richmond, VA 23284, USA.}, author = {Rudd, M. L. and Nicolas, A. N. and Brown, B. L. and Fischer-Stenger, K. and Stewart, J. K.}, citeulike-article-id = {3182989}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.jneuroim.2004.10.013}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/15652409}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=15652409}, doi = {10.1016/j.jneuroim.2004.10.013}, issn = {0165-5728}, journal = {Journal of neuroimmunology}, keywords = {macrophages, serotonin, transporter}, month = {February}, number = {1-2}, pages = {113--118}, posted-at = {2008-09-02 21:47:53}, priority = {3}, title = {Peritoneal macrophages express the serotonin transporter.}, url = {http://dx.doi.org/10.1016/j.jneuroim.2004.10.013}, volume = {159}, year = {2005} }

TY - JOUR ID - citeulike:3182989 L3 - citeulike-article-id:3182989 N2 - Although it is known that macrophages take up serotonin, a specific monoamine transporter has not been identified in macrophages. In this study, mRNA coding for the serotonin transporter (SERT) was detected with the reverse transcription-polymerase chain reaction (RT-PCR) in recruited mouse peritoneal macrophages. Sequencing confirmed the identity of the RT-PCR product to mouse SERT mRNA. SERT protein was detected by Western blotting. Macrophage activation with lipopolysaccharide had no effect on expression of SERT mRNA or protein. Consistent with expression of a functional SERT, specific uptake of (3)H-serotonin in macrophages was sodium dependent and inhibited by fluoxetine (IC(50) 6.9 nM) and desipramine (IC(50) 32 nM) but not by nisoxetine or reserpine. IS - 1-2 JF - Journal of neuroimmunology SN - 0165-5728 AD - Department of Biology, Virginia Commonwealth University, Richmond, VA 23284, USA. EP - 118 TI - Peritoneal macrophages express the serotonin transporter. VL - 159 SP - 113 KW - macrophages KW - serotonin KW - transporter AU - Rudd, ML AU - Nicolas, AN AU - Brown, BL AU - Fischer-Stenger, K AU - Stewart, JK PY - 2005/02// UR - http://dx.doi.org/10.1016/j.jneuroim.2004.10.013 ER -