Expression of a high-affinity serotonin transporter in human lymphocytes.

@article{citeulike:3194363, abstract = {This study was undertaken to assess the capability of lymphocytes to actively transport serotonin (5-HT). The data we obtained showed that lymphocytes isolated from the blood of normal human subjects contained a high-affinity uptake system for [3H]5-HT. Kinetic analysis of the uptake data as computed by regression analysis from Lineweaver--Burk plots, yielded a Km of 180 +/- 20 nM and Vmax of 94 +/- pmole/10(7) cells. The uptake of [3H]5-HT was temperature, sodium and chloride dependent and was potently inhibited by the antidepressants clomipramine, imipramine, fluoxetine and fluvoxamine, which are specific for the 5-HT transporter. Compounds that are more selective for norepinephrine and dopamine transporters such as mazindol, desipramine, and GBR 19209 had a lower inhibitory effect on the uptake of [3H]5-HT in human lymphocytes. The expression of a 5-HT transporter in human lymphocytes that resembles 5-HT uptake by platelets and brain synaptosomes may provide insights into the potential role of 5-HT in immune function and its relationship to the neurobiology of affective and addictive disorders.}, address = {Department of Radiology (Division of Nuclear Medicine), Emory University School of Medicine, Atlanta, GA 30322.}, author = {Faraj, B. A. and Olkowski, Z. L. and Jackson, R. T.}, citeulike-article-id = {3194363}, issn = {0192-0561}, journal = {International journal of immunopharmacology}, keywords = {lymphocyte, sert}, month = {July}, number = {7}, pages = {561--567}, posted-at = {2008-09-04 16:59:53}, priority = {5}, title = {Expression of a high-affinity serotonin transporter in human lymphocytes.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/7928004}, volume = {16}, year = {1994} }

TY - JOUR ID - citeulike:3194363 L3 - citeulike-article-id:3194363 N2 - This study was undertaken to assess the capability of lymphocytes to actively transport serotonin (5-HT). The data we obtained showed that lymphocytes isolated from the blood of normal human subjects contained a high-affinity uptake system for [3H]5-HT. Kinetic analysis of the uptake data as computed by regression analysis from Lineweaver--Burk plots, yielded a Km of 180 +/- 20 nM and Vmax of 94 +/- pmole/10(7) cells. The uptake of [3H]5-HT was temperature, sodium and chloride dependent and was potently inhibited by the antidepressants clomipramine, imipramine, fluoxetine and fluvoxamine, which are specific for the 5-HT transporter. Compounds that are more selective for norepinephrine and dopamine transporters such as mazindol, desipramine, and GBR 19209 had a lower inhibitory effect on the uptake of [3H]5-HT in human lymphocytes. The expression of a 5-HT transporter in human lymphocytes that resembles 5-HT uptake by platelets and brain synaptosomes may provide insights into the potential role of 5-HT in immune function and its relationship to the neurobiology of affective and addictive disorders. IS - 7 JF - International journal of immunopharmacology SN - 0192-0561 AD - Department of Radiology (Division of Nuclear Medicine), Emory University School of Medicine, Atlanta, GA 30322. EP - 567 TI - Expression of a high-affinity serotonin transporter in human lymphocytes. VL - 16 SP - 561 KW - lymphocyte KW - sert AU - Faraj, BA AU - Olkowski, ZL AU - Jackson, RT PY - 1994/07// UR - http://view.ncbi.nlm.nih.gov/pubmed/7928004 ER -