Presence and characterization of the serotonin transporter in human resting lymphocytes.

@article{citeulike:3194369, abstract = {Although evidence exists of the presence of a serotonin (5-HT) reuptake system in lymphocytes, no information is available on the pharmacological characterization of this structure. Our study aimed to investigate this matter, therefore, by means of the binding of [3H]-paroxetine ([3H]PAR), a selective 5-HT reuptake inhibitor (SSRI), which is considered the ligand of choice for binding studies. Lymphocytes were obtained from a pool of 20 healthy subjects who volunteered for the study. The results showed the presence of a specific and saturable [3H]PAR binding to lymphocyte membranes, with a Hill number close to unity indicative of the presence of one site only. The most potent drugs inhibiting [3H]PAR binding were SSRIs (paroxetine, fluoxetine, citalopram) followed by clomipramine, imipramine, and 5-HT, whereas haloperidol, mazindol, and nomifensine had a negligible effect. These findings suggest that [3H]-PAR in human resting lymphocytes specifically labels the 5-HT transporter.}, address = {Dipartimento di Psichiatria, Neurobiologia, Farmacologia e Biotecnologie, University of Pisa, Italy.}, author = {Marazziti, D. and Rossi, A. and Giannaccini, G. and Baroni, S. and Lucacchini, A. and Cassano, G. B.}, citeulike-article-id = {3194369}, doi = {10.1016/S0893-133X(97)00204-2}, issn = {0893-133X}, journal = {Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology}, keywords = {lymphocyte, sert}, month = {August}, number = {2}, pages = {154--159}, posted-at = {2008-09-04 17:02:45}, priority = {5}, title = {Presence and characterization of the serotonin transporter in human resting lymphocytes.}, url = {http://dx.doi.org/10.1016/S0893-133X(97)00204-2}, volume = {19}, year = {1998} }

TY - JOUR ID - citeulike:3194369 L3 - citeulike-article-id:3194369 N2 - Although evidence exists of the presence of a serotonin (5-HT) reuptake system in lymphocytes, no information is available on the pharmacological characterization of this structure. Our study aimed to investigate this matter, therefore, by means of the binding of [3H]-paroxetine ([3H]PAR), a selective 5-HT reuptake inhibitor (SSRI), which is considered the ligand of choice for binding studies. Lymphocytes were obtained from a pool of 20 healthy subjects who volunteered for the study. The results showed the presence of a specific and saturable [3H]PAR binding to lymphocyte membranes, with a Hill number close to unity indicative of the presence of one site only. The most potent drugs inhibiting [3H]PAR binding were SSRIs (paroxetine, fluoxetine, citalopram) followed by clomipramine, imipramine, and 5-HT, whereas haloperidol, mazindol, and nomifensine had a negligible effect. These findings suggest that [3H]-PAR in human resting lymphocytes specifically labels the 5-HT transporter. IS - 2 JF - Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology SN - 0893-133X AD - Dipartimento di Psichiatria, Neurobiologia, Farmacologia e Biotecnologie, University of Pisa, Italy. EP - 159 TI - Presence and characterization of the serotonin transporter in human resting lymphocytes. VL - 19 SP - 154 KW - lymphocyte KW - sert AU - Marazziti, D AU - Rossi, A AU - Giannaccini, G AU - Baroni, S AU - Lucacchini, A AU - Cassano, GB PY - 1998/08// UR - http://dx.doi.org/10.1016/S0893-133X(97)00204-2 ER -