Identification of dopamine plasma membrane and vesicular transporters in human peripheral blood lymphocytes. Export

@article{citeulike:3194438, abstract = {Plasma membrane dopamine transporter (DAT), vesicular monoamine transporters (VMAT) type-1 and -2 and the expression of the dopaminergic markers dopamine and tyrosine hydroxylase were assessed in membranes and/or in cytospin centrifuged human peripheral blood lymphocytes. The radiolabeled DAT ligand [3H]GBR12935 was bound to peripheral lymphocytes in a manner consistent with the specific binding to a dopamine uptake system, with a dissociation constant similar to that found in striatum, but with a lower density of binding sites. On the other hand, no specific binding occurred in cerebellum used as a test tissue not expressing DAT. Western blot analysis using antibodies raised against amino or carboxy terminus of DAT or against VMAT-1 or VMAT-2 revealed labeling of single bands of approximately 76, 55 or 68 KDa, respectively, displaying similar migration characteristics in lymphocytes and test tissues used for comparison. Immunofluorescence revealed that anti-dopamine, anti-tyrosine hydroxylase, anti-DAT, anti-VMAT-1 and anti-VMAT-2 antibodies labeled the total population of cytospin-centrifuged lymphocytes mounted on microscope slides. Confocal laser microscopy demonstrated that dopamine and VMAT-2 immunoreactivity was developed mainly in cytoplasmic punctiform areas likely corresponding to vesicles and to a lower extent was associated to plasma membrane. Tyrosine hydroxylase immunoreactivity was diffused to cytoplasm and to plasma membrane of lymphocytes, whereas DAT and VMAT-1 immunoreactivity were located almost exclusively in lymphocyte plasma membrane and cytoplasm, respectively. Lymphocyte DAT characterized in this study has probably functional relevance as [3H]dopamine was taken up by intact lymphocytes and uptake was inhibited specifically by compounds known to affect dopamine transport. These findings indicate that human peripheral blood lymphocytes possess DAT plasma membrane and VMAT-1 and VMAT-2 transporters. Increasing evidence indicates that dopamine transporter changes may be related to neuronal injury. In view of this assessment of lymphocyte DAT and VMAT transporters can be considered for identifying pathologies characterized by impaired dopaminergic neurotransmission.}, address = {Sezione di Anatomia Umana, Dipartimento di Scienze Farmacologiche e Medicina Sperimentale, Universit\`{a} di Camerino, Via Scalzino, 3, 62032, Camerino, Italy. amenta@cambio.unicam.it}, author = {Amenta, F. and Bronzetti, E. and Cantalamessa, F. and El-Assouad, D. and Felici, L. and Ricci, A. and Tayebati, S. K.}, citeulike-article-id = {3194438}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/11431013}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=11431013}, day = {2}, issn = {0165-5728}, journal = {Journal of neuroimmunology}, keywords = {dat, lymphocyte, pbmc}, month = {July}, number = {1-2}, pages = {133--142}, posted-at = {2008-09-04 17:46:58}, priority = {2}, title = {Identification of dopamine plasma membrane and vesicular transporters in human peripheral blood lymphocytes.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/11431013}, volume = {117}, year = {2001} }

TY - JOUR ID - citeulike:3194438 L3 - citeulike-article-id:3194438 N2 - Plasma membrane dopamine transporter (DAT), vesicular monoamine transporters (VMAT) type-1 and -2 and the expression of the dopaminergic markers dopamine and tyrosine hydroxylase were assessed in membranes and/or in cytospin centrifuged human peripheral blood lymphocytes. The radiolabeled DAT ligand [3H]GBR12935 was bound to peripheral lymphocytes in a manner consistent with the specific binding to a dopamine uptake system, with a dissociation constant similar to that found in striatum, but with a lower density of binding sites. On the other hand, no specific binding occurred in cerebellum used as a test tissue not expressing DAT. Western blot analysis using antibodies raised against amino or carboxy terminus of DAT or against VMAT-1 or VMAT-2 revealed labeling of single bands of approximately 76, 55 or 68 KDa, respectively, displaying similar migration characteristics in lymphocytes and test tissues used for comparison. Immunofluorescence revealed that anti-dopamine, anti-tyrosine hydroxylase, anti-DAT, anti-VMAT-1 and anti-VMAT-2 antibodies labeled the total population of cytospin-centrifuged lymphocytes mounted on microscope slides. Confocal laser microscopy demonstrated that dopamine and VMAT-2 immunoreactivity was developed mainly in cytoplasmic punctiform areas likely corresponding to vesicles and to a lower extent was associated to plasma membrane. Tyrosine hydroxylase immunoreactivity was diffused to cytoplasm and to plasma membrane of lymphocytes, whereas DAT and VMAT-1 immunoreactivity were located almost exclusively in lymphocyte plasma membrane and cytoplasm, respectively. Lymphocyte DAT characterized in this study has probably functional relevance as [3H]dopamine was taken up by intact lymphocytes and uptake was inhibited specifically by compounds known to affect dopamine transport. These findings indicate that human peripheral blood lymphocytes possess DAT plasma membrane and VMAT-1 and VMAT-2 transporters. Increasing evidence indicates that dopamine transporter changes may be related to neuronal injury. In view of this assessment of lymphocyte DAT and VMAT transporters can be considered for identifying pathologies characterized by impaired dopaminergic neurotransmission. IS - 1-2 JF - Journal of neuroimmunology SN - 0165-5728 AD - Sezione di Anatomia Umana, Dipartimento di Scienze Farmacologiche e Medicina Sperimentale, Università di Camerino, Via Scalzino, 3, 62032, Camerino, Italy. amenta@cambio.unicam.it EP - 142 TI - Identification of dopamine plasma membrane and vesicular transporters in human peripheral blood lymphocytes. VL - 117 SP - 133 KW - dat KW - lymphocyte KW - pbmc AU - Amenta, F AU - Bronzetti, E AU - Cantalamessa, F AU - El-Assouad, D AU - Felici, L AU - Ricci, A AU - Tayebati, SK PY - 2001/07/02/ UR - http://view.ncbi.nlm.nih.gov/pubmed/11431013 ER -