Expression of the dopamine transporter gene is regulated by the 3' UTR VNTR: Evidence from brain and lymphocytes using quantitative RT-PCR. Export

@article{citeulike:3194452, abstract = {Genetic association studies provide considerable evidence that the 10-repeat allele of a variable number tandem repeat (VNTR) in the 3'-untranslated region (3'-UTR) of the dopamine transporter gene (DAT1) is associated with a range of psychiatric phenotypes, most notably, attention deficit hyperactivity disorder. The mechanism for this association is not yet understood, although several lines of evidence implicate variation in gene expression. In this study, we measured DAT1 messenger RNA levels in cerebellum, temporal lobe, and lymphocytes using quantitative real-time reverse-transcription polymerase chain reaction. Relative to a set of four control housekeeping genes (beta-actin, GAPD, ribosomal 18S, and beta2-microglobulin) we observed that increased levels of DAT1 expression were associated with the number of 10-repeat alleles. These data provide direct evidence that the VNTR, or another polymorphism in linkage disequilibrium with the VNTR, is involved in regulating expression of this gene.}, address = {Social, Genetic, and Developmental Research Centre, Institute of Psychiatry, De Crespigny Park, Denmark Hill, London SE5 8AF, United Kingdom. j.mill@iop.kcl.ac.uk}, author = {Mill, J. and Asherson, P. and Browes, C. and D'Souza, U. and Craig, I.}, citeulike-article-id = {3194452}, citeulike-linkout-0 = {http://dx.doi.org/10.1002/ajmg.b.10948}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/12457396}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=12457396}, day = {8}, doi = {10.1002/ajmg.b.10948}, issn = {0148-7299}, journal = {American journal of medical genetics}, keywords = {dat, gene, lymphocyte, rt-pcr}, month = {December}, number = {8}, pages = {975--979}, posted-at = {2008-09-04 17:51:01}, priority = {2}, title = {Expression of the dopamine transporter gene is regulated by the 3' UTR VNTR: Evidence from brain and lymphocytes using quantitative RT-PCR.}, url = {http://dx.doi.org/10.1002/ajmg.b.10948}, volume = {114}, year = {2002} }

TY - JOUR ID - citeulike:3194452 L3 - citeulike-article-id:3194452 N2 - Genetic association studies provide considerable evidence that the 10-repeat allele of a variable number tandem repeat (VNTR) in the 3'-untranslated region (3'-UTR) of the dopamine transporter gene (DAT1) is associated with a range of psychiatric phenotypes, most notably, attention deficit hyperactivity disorder. The mechanism for this association is not yet understood, although several lines of evidence implicate variation in gene expression. In this study, we measured DAT1 messenger RNA levels in cerebellum, temporal lobe, and lymphocytes using quantitative real-time reverse-transcription polymerase chain reaction. Relative to a set of four control housekeeping genes (beta-actin, GAPD, ribosomal 18S, and beta2-microglobulin) we observed that increased levels of DAT1 expression were associated with the number of 10-repeat alleles. These data provide direct evidence that the VNTR, or another polymorphism in linkage disequilibrium with the VNTR, is involved in regulating expression of this gene. IS - 8 JF - American journal of medical genetics SN - 0148-7299 AD - Social, Genetic, and Developmental Research Centre, Institute of Psychiatry, De Crespigny Park, Denmark Hill, London SE5 8AF, United Kingdom. j.mill@iop.kcl.ac.uk EP - 979 TI - Expression of the dopamine transporter gene is regulated by the 3' UTR VNTR: Evidence from brain and lymphocytes using quantitative RT-PCR. VL - 114 SP - 975 KW - dat KW - gene KW - lymphocyte KW - rt-pcr AU - Mill, J AU - Asherson, P AU - Browes, C AU - D'Souza, U AU - Craig, I PY - 2002/12/08/ UR - http://dx.doi.org/10.1002/ajmg.b.10948 ER -