Can serotonin transporter genotype predict serotonergic function, chronicity, and severity of drinking? Export

@article{citeulike:3388080, abstract = {Serotonin transporter (5-HTT) activity is greater in carriers of the long (L) vs. short (S) alleles of the 5-HTT-linked polymorphic region (5'-HTTLPR) among healthy control subjects but not alcohol-dependent adults. In 198 alcoholics, we determined the relationship between current or lifetime drinking and platelet 5-HTT function and density among allelic variants of the 5'-HTTLPR. SS subjects were younger than L-carriers (LL and LS) (p<0.0085) and had fewer years of lifetime drinking. For L-carriers, the mean of Bmax for paroxetine binding, but not Vmax for serotonin (5-HT) uptake, was lower than that for SS subjects (p<0.05). More L-carriers than their SS counterparts had Vmax for 5-HT uptake below 200 nmol/10(7) platelets-min (p<0.05) and Bmax for paroxetine binding below 600 nmol/mg protein (p<0.06). Current drinking (drinks per day during the past 14 days) correlated positively with Km and Vmax of platelet 5-HT uptake (p<0.05) and negatively with Bmax, but not Kd, of paroxetine binding (p<0.05) for L-carriers alone. Years of lifetime drinking correlated negatively with Km and Vmax of platelet 5-HT uptake (p<0.05) and B(max), but not Kd, of paroxetine binding (p<0.05) for L-carriers alone. Among L-carriers alone, there were higher levels of platelet 5-HT uptake and lower levels of platelet paroxetine binding with increased drinking, and more lifetime drinking was associated with modestly lower levels of 5-HT uptake and paroxetine binding. Thus, 5-HTT expression varies with current and lifetime drinking in L-carriers alone.}, author = {Johnson, B. A. and Javors, M. A. and Roache, J. D. and Seneviratne, C. and Bergeson, S. E. and Ait-Daoud, N. and Dawes, M. A. and Ma, J. Z.}, citeulike-article-id = {3388080}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.pnpbp.2007.07.030}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/17950969}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=17950969}, comment = {Looked at blood platelets from alcoholic persons. They did binding assay to calculate Kd, Km, Vmax, Bmax. Group LS and LL together In L carriers Bmax is lower than in SS (p<0.005) while Vmax is not significantly different in alcoholics. There hypothesis is L carriers will have higher toxicity. }, day = {1}, doi = {10.1016/j.pnpbp.2007.07.030}, issn = {0278-5846}, journal = {Progress in neuro-psychopharmacology \& biological psychiatry}, keywords = {5-httlpr, achem, platelets}, month = {January}, number = {1}, pages = {209--216}, posted-at = {2008-10-08 18:43:06}, priority = {5}, title = {Can serotonin transporter genotype predict serotonergic function, chronicity, and severity of drinking?}, url = {http://dx.doi.org/10.1016/j.pnpbp.2007.07.030}, volume = {32}, year = {2008} }

TY - JOUR ID - citeulike:3388080 L3 - citeulike-article-id:3388080 N2 - Serotonin transporter (5-HTT) activity is greater in carriers of the long (L) vs. short (S) alleles of the 5-HTT-linked polymorphic region (5'-HTTLPR) among healthy control subjects but not alcohol-dependent adults. In 198 alcoholics, we determined the relationship between current or lifetime drinking and platelet 5-HTT function and density among allelic variants of the 5'-HTTLPR. SS subjects were younger than L-carriers (LL and LS) (p<0.0085) and had fewer years of lifetime drinking. For L-carriers, the mean of Bmax for paroxetine binding, but not Vmax for serotonin (5-HT) uptake, was lower than that for SS subjects (p<0.05). More L-carriers than their SS counterparts had Vmax for 5-HT uptake below 200 nmol/10(7) platelets-min (p<0.05) and Bmax for paroxetine binding below 600 nmol/mg protein (p<0.06). Current drinking (drinks per day during the past 14 days) correlated positively with Km and Vmax of platelet 5-HT uptake (p<0.05) and negatively with Bmax, but not Kd, of paroxetine binding (p<0.05) for L-carriers alone. Years of lifetime drinking correlated negatively with Km and Vmax of platelet 5-HT uptake (p<0.05) and B(max), but not Kd, of paroxetine binding (p<0.05) for L-carriers alone. Among L-carriers alone, there were higher levels of platelet 5-HT uptake and lower levels of platelet paroxetine binding with increased drinking, and more lifetime drinking was associated with modestly lower levels of 5-HT uptake and paroxetine binding. Thus, 5-HTT expression varies with current and lifetime drinking in L-carriers alone. IS - 1 JF - Progress in neuro-psychopharmacology & biological psychiatry SN - 0278-5846 EP - 216 TI - Can serotonin transporter genotype predict serotonergic function, chronicity, and severity of drinking? VL - 32 SP - 209 KW - 5-httlpr KW - achem KW - platelets N1 - Looked at blood platelets from alcoholic persons. They did binding assay to calculate Kd, Km, Vmax, Bmax. Group LS and LL together In L carriers Bmax is lower than in SS (p<0.005) while Vmax is not significantly different in alcoholics. There hypothesis is L carriers will have higher toxicity. AU - Johnson, BA AU - Javors, MA AU - Roache, JD AU - Seneviratne, C AU - Bergeson, SE AU - Ait-Daoud, N AU - Dawes, MA AU - Ma, JZ PY - 2008/01/01/ UR - http://dx.doi.org/10.1016/j.pnpbp.2007.07.030 ER -