Deficiency in a cytosolic ribose-5-phosphate isomerase causes chloroplast dysfunction, late flowering and premature cell death in Arabidopsis. Export

@article{citeulike:5976331, abstract = {The oxidative pentose phosphate pathway (oxPPP) is part of central metabolism, consisting of two distinct phases: the oxidative phase and the non-oxidative phase. The non-oxidative phase of the oxPPP generates carbon skeletons for the synthesis of nucleotides, aromatic amino acids, phenylpropanoids and their derivatives, which are essential for plant growth and development. However, it is not well understood how the non-oxidative phase of the oxPPP contributes to plant growth and development. Here, we report the characterization of Arabidopsis T-DNA knockout mutants of the RPI2 gene (At2g01290), which encodes a cytosolic ribose-5-phosphate isomerase (RPI) that catalyzes the reversible interconversion of ribulose-5-phosphate and ribose-5-phosphate in the non-oxidative phase of the oxPPP. Although recombinant Arabidopsis RPI2 protein exhibits marked RPI enzymatic activity, knockout of the RPI2 gene does not significantly change the total RPI activity in the mutant plants. Interestingly, knockout of RPI2 interferes with chloroplast structure and decreases chloroplast photosynthetic capacity. The rpi2 mutants accumulate less starch in the leaves and flower significantly later than wild-type when grown under short-day conditions. Furthermore, the rpi2 mutants display premature cell death in the leaves when grown at an above-normal temperature (26 degrees C). These results demonstrate that a deficiency in the non-oxidative phase of the cytosolic oxPPP has pleiotropic effects on plant growth and development and causes premature cell death.}, author = {Xiong, Yuqing and DeFraia, Christopher and Williams, Donna and Zhang, Xudong and Mou, Zhonglin}, citeulike-article-id = {5976331}, citeulike-linkout-0 = {http://www.biomedexperts.com/Abstract.bme/19744161}, citeulike-linkout-1 = {http://dx.doi.org/10.1111/j.1399-3054.2009.01276.x}, citeulike-linkout-2 = {http://www.ingentaconnect.com/content/mksg/ppl/2009/00000137/00000003/art00005}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/19744161}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=19744161}, doi = {10.1111/j.1399-3054.2009.01276.x}, issn = {1399-3054}, journal = {Physiologia plantarum}, keywords = {aromatic\_amino\_acids, carbon\_skeletons, central\_metabolism, characterization, cytosolic, derivatives, enzymatic\_activity, growth\_and\_development, interconversion, knockout, mutant\_plants, mutants, normal\_temperature, phosphate, photosynthetic\_capacity, pleiotropic\_effects, rpi, starch}, month = {November}, number = {3}, pages = {249--263}, posted-at = {2009-11-08 21:55:21}, priority = {2}, publisher = {Blackwell Publishing}, title = {Deficiency in a cytosolic ribose-5-phosphate isomerase causes chloroplast dysfunction, late flowering and premature cell death in Arabidopsis.}, url = {http://dx.doi.org/10.1111/j.1399-3054.2009.01276.x}, volume = {137}, year = {2009} }

TY - JOUR ID - citeulike:5976331 L3 - citeulike-article-id:5976331 N2 - The oxidative pentose phosphate pathway (oxPPP) is part of central metabolism, consisting of two distinct phases: the oxidative phase and the non-oxidative phase. The non-oxidative phase of the oxPPP generates carbon skeletons for the synthesis of nucleotides, aromatic amino acids, phenylpropanoids and their derivatives, which are essential for plant growth and development. However, it is not well understood how the non-oxidative phase of the oxPPP contributes to plant growth and development. Here, we report the characterization of Arabidopsis T-DNA knockout mutants of the RPI2 gene (At2g01290), which encodes a cytosolic ribose-5-phosphate isomerase (RPI) that catalyzes the reversible interconversion of ribulose-5-phosphate and ribose-5-phosphate in the non-oxidative phase of the oxPPP. Although recombinant Arabidopsis RPI2 protein exhibits marked RPI enzymatic activity, knockout of the RPI2 gene does not significantly change the total RPI activity in the mutant plants. Interestingly, knockout of RPI2 interferes with chloroplast structure and decreases chloroplast photosynthetic capacity. The rpi2 mutants accumulate less starch in the leaves and flower significantly later than wild-type when grown under short-day conditions. Furthermore, the rpi2 mutants display premature cell death in the leaves when grown at an above-normal temperature (26 degrees C). These results demonstrate that a deficiency in the non-oxidative phase of the cytosolic oxPPP has pleiotropic effects on plant growth and development and causes premature cell death. IS - 3 JF - Physiologia plantarum SN - 1399-3054 EP - 263 TI - Deficiency in a cytosolic ribose-5-phosphate isomerase causes chloroplast dysfunction, late flowering and premature cell death in Arabidopsis. PB - Blackwell Publishing VL - 137 SP - 249 KW - aromatic_amino_acids KW - carbon_skeletons KW - central_metabolism KW - characterization KW - cytosolic KW - derivatives KW - enzymatic_activity KW - growth_and_development KW - interconversion KW - knockout KW - mutant_plants KW - mutants KW - normal_temperature KW - phosphate KW - photosynthetic_capacity KW - pleiotropic_effects KW - rpi KW - starch AU - Xiong, Yuqing AU - DeFraia, Christopher AU - Williams, Donna AU - Zhang, Xudong AU - Mou, Zhonglin PY - 2009/11// UR - http://dx.doi.org/10.1111/j.1399-3054.2009.01276.x ER -