Sorting single molecules: application to diagnostics and evolutionary biotechnology.

@article{citeulike:2632617, abstract = {A method is described that provides for detection and identification of single molecules in solution. The method is based on fluorescence correlation spectroscopy, which records spatio-temporal correlations among fluctuating light signals, coupled with devices for trapping single molecules in an electric field. This technique is applied to studies of molecular evolution, where it allows fast screening of large mutant spectra in which targets are labeled by specific fluorescent ligands. The method expands the horizon in molecular diagnostics by making it possible to monitor concentrations down to (less than) 10(-15) M without any need for amplification.}, address = {Max-Planck-Institut f\"{u}r Biophysikalische Chemie, G\"{o}ttingen, Federal Republic of Germany.}, author = {Eigen, M. and Rigler, R. }, citeulike-article-id = {2632617}, issn = {0027-8424}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, keywords = {classic, fcs, single\_molecule}, month = {June}, number = {13}, pages = {5740--5747}, posted-at = {2008-04-05 15:16:49}, priority = {4}, title = {Sorting single molecules: application to diagnostics and evolutionary biotechnology.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/7517036}, volume = {91}, year = {1994} }

TY - JOUR ID - citeulike:2632617 L3 - citeulike-article-id:2632617 TI - Sorting single molecules: application to diagnostics and evolutionary biotechnology. JF - Proceedings of the National Academy of Sciences of the United States of America VL - 91 IS - 13 SP - 5740 EP - 5747 AD - Max-Planck-Institut für Biophysikalische Chemie, Göttingen, Federal Republic of Germany. SN - 0027-8424 N2 - A method is described that provides for detection and identification of single molecules in solution. The method is based on fluorescence correlation spectroscopy, which records spatio-temporal correlations among fluctuating light signals, coupled with devices for trapping single molecules in an electric field. This technique is applied to studies of molecular evolution, where it allows fast screening of large mutant spectra in which targets are labeled by specific fluorescent ligands. The method expands the horizon in molecular diagnostics by making it possible to monitor concentrations down to (less than) 10(-15) M without any need for amplification. KW - classic KW - fcs KW - single_molecule AU - Eigen, M AU - Rigler, R PY - 1994/06/21/ UR - http://view.ncbi.nlm.nih.gov/pubmed/7517036 ER -