Tetracycline aptamer-controlled regulation of pre-mRNA splicing in yeast Export

@article{citeulike:4401637, abstract = {Splicing of pre-mRNA is a critical step in mRNA maturation and disturbances cause several genetic disorders. We apply the synthetic tetracycline (tc)-binding riboswitch to establish a gene expression system for conditional tc-dependent control of pre-mRNA splicing in yeast. Efficient regulation is obtained when the aptamer is inserted close to the 5'splice site (SS) with the consensus sequence of the SS located within the aptamer stem. Structural probing indicates limited spontaneous cleavage within this stem in the absence of the ligand. Addition of tc leads to tightening of the stem and the whole aptamer structure which probably prevents recognition of the 5'SS. Combination of more then one aptamer-regulated intron increases the extent of regulation leading to highly efficient conditional gene expression systems. Our findings highlight the potential of direct RNA-ligand interaction for regulation of gene expression. 10.1093/nar/gkm425}, author = {Weigand, Julia E. and Suess, Beatrix}, citeulike-article-id = {4401637}, citeulike-linkout-0 = {http://dx.doi.org/10.1093/nar/gkm425}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/17567606}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=17567606}, day = {12}, doi = {10.1093/nar/gkm425}, journal = {Nucl. Acids Res.}, keywords = {riboswitch, rna}, month = {June}, pages = {gkm425+}, posted-at = {2009-04-24 20:54:23}, priority = {2}, title = {Tetracycline aptamer-controlled regulation of pre-mRNA splicing in yeast}, url = {http://dx.doi.org/10.1093/nar/gkm425}, year = {2007} }

TY - JOUR ID - citeulike:4401637 L3 - citeulike-article-id:4401637 N2 - Splicing of pre-mRNA is a critical step in mRNA maturation and disturbances cause several genetic disorders. We apply the synthetic tetracycline (tc)-binding riboswitch to establish a gene expression system for conditional tc-dependent control of pre-mRNA splicing in yeast. Efficient regulation is obtained when the aptamer is inserted close to the 5'splice site (SS) with the consensus sequence of the SS located within the aptamer stem. Structural probing indicates limited spontaneous cleavage within this stem in the absence of the ligand. Addition of tc leads to tightening of the stem and the whole aptamer structure which probably prevents recognition of the 5'SS. Combination of more then one aptamer-regulated intron increases the extent of regulation leading to highly efficient conditional gene expression systems. Our findings highlight the potential of direct RNA-ligand interaction for regulation of gene expression. 10.1093/nar/gkm425 JF - Nucl. Acids Res. TI - Tetracycline aptamer-controlled regulation of pre-mRNA splicing in yeast SP - gkm425 KW - riboswitch KW - rna AU - Weigand, Julia AU - Suess, Beatrix PY - 2007/06/12/ UR - http://dx.doi.org/10.1093/nar/gkm425 ER -