Electron microscopy of whole cells in liquid with nanometer resolution. Export

@article{citeulike:4067313, abstract = {Single gold-tagged epidermal growth factor (EGF) molecules bound to cellular EGF receptors of fixed fibroblast cells were imaged in liquid with a scanning transmission electron microscope (STEM). The cells were placed in buffer solution in a microfluidic device with electron transparent windows inside the vacuum of the electron microscope. A spatial resolution of 4 nm and a pixel dwell time of 20 micros were obtained. The liquid layer was sufficiently thick to contain the cells with a thickness of 7 +/- 1 microm. The experimental findings are consistent with a theoretical calculation. Liquid STEM is a unique approach for imaging single molecules in whole cells with significantly improved resolution and imaging speed over existing methods.}, author = {de Jonge, N. and Peckys, D. B. and Kremers, G. J. and Piston, D. W.}, citeulike-article-id = {4067313}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0809567106}, citeulike-linkout-1 = {http://www.pnas.org/content/106/7/2159.short.abstract}, citeulike-linkout-2 = {http://www.pnas.org/content/106/7/2159.short.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/19164524}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=19164524}, day = {17}, doi = {10.1073/pnas.0809567106}, issn = {1091-6490}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, keywords = {dtc, em, project}, month = {February}, number = {7}, pages = {2159--2164}, posted-at = {2009-11-05 11:13:16}, priority = {2}, title = {Electron microscopy of whole cells in liquid with nanometer resolution.}, url = {http://dx.doi.org/10.1073/pnas.0809567106}, volume = {106}, year = {2009} }

TY - JOUR ID - citeulike:4067313 L3 - citeulike-article-id:4067313 N2 - Single gold-tagged epidermal growth factor (EGF) molecules bound to cellular EGF receptors of fixed fibroblast cells were imaged in liquid with a scanning transmission electron microscope (STEM). The cells were placed in buffer solution in a microfluidic device with electron transparent windows inside the vacuum of the electron microscope. A spatial resolution of 4 nm and a pixel dwell time of 20 micros were obtained. The liquid layer was sufficiently thick to contain the cells with a thickness of 7 +/- 1 microm. The experimental findings are consistent with a theoretical calculation. Liquid STEM is a unique approach for imaging single molecules in whole cells with significantly improved resolution and imaging speed over existing methods. IS - 7 JF - Proceedings of the National Academy of Sciences of the United States of America SN - 1091-6490 EP - 2164 TI - Electron microscopy of whole cells in liquid with nanometer resolution. VL - 106 SP - 2159 KW - dtc KW - em KW - project AU - de Jonge, N AU - Peckys, DB AU - Kremers, GJ AU - Piston, DW PY - 2009/02/17/ UR - http://dx.doi.org/10.1073/pnas.0809567106 ER -