Attenuation of LDH-A expression uncovers a link between glycolysis, mitochondrial physiology, and tumor maintenance. Export

@article{citeulike:4634173, abstract = {Alterations in cellular metabolism are among the most consistent hallmarks of cancer. Herein we have investigated the relationship between increased aerobic lactate production and mitochondrial physiology in tumor cells. To diminish the ability of malignant cells to metabolize pyruvate to lactate, lactate dehydrogenase A (LDH-A) levels were knocked down by means of LDH-A short hairpin RNAs. Reduction in LDH-A activity resulted in stimulation of mitochondrial respiration and decrease of mitochondrial membrane potential. It also compromised the ability of these tumor cells to proliferate under hypoxia. The tumorigenicity of the LDH-A-deficient cells was severely diminished, and this phenotype was reversed by complementation with the human ortholog LDH-A protein. These results demonstrate that LDH-A plays a key role in tumor maintenance.}, author = {Fantin, V. R. and St-Pierre, J. and Leder, P.}, citeulike-article-id = {4634173}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.ccr.2006.04.023}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/16766262}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=16766262}, doi = {10.1016/j.ccr.2006.04.023}, issn = {1535-6108}, journal = {Cancer cell}, keywords = {cancer, glycolysis, lhda, metabolism, tumor}, month = {June}, number = {6}, pages = {425--434}, posted-at = {2009-05-26 00:48:34}, priority = {2}, title = {Attenuation of LDH-A expression uncovers a link between glycolysis, mitochondrial physiology, and tumor maintenance.}, url = {http://dx.doi.org/10.1016/j.ccr.2006.04.023}, volume = {9}, year = {2006} }

TY - JOUR ID - citeulike:4634173 L3 - citeulike-article-id:4634173 N2 - Alterations in cellular metabolism are among the most consistent hallmarks of cancer. Herein we have investigated the relationship between increased aerobic lactate production and mitochondrial physiology in tumor cells. To diminish the ability of malignant cells to metabolize pyruvate to lactate, lactate dehydrogenase A (LDH-A) levels were knocked down by means of LDH-A short hairpin RNAs. Reduction in LDH-A activity resulted in stimulation of mitochondrial respiration and decrease of mitochondrial membrane potential. It also compromised the ability of these tumor cells to proliferate under hypoxia. The tumorigenicity of the LDH-A-deficient cells was severely diminished, and this phenotype was reversed by complementation with the human ortholog LDH-A protein. These results demonstrate that LDH-A plays a key role in tumor maintenance. IS - 6 JF - Cancer cell SN - 1535-6108 EP - 434 TI - Attenuation of LDH-A expression uncovers a link between glycolysis, mitochondrial physiology, and tumor maintenance. VL - 9 SP - 425 KW - cancer KW - glycolysis KW - lhda KW - metabolism KW - tumor AU - Fantin, VR AU - St-Pierre, J AU - Leder, P PY - 2006/06// UR - http://dx.doi.org/10.1016/j.ccr.2006.04.023 ER -