The genome of HSV-1 translocates through the nuclear pore as a condensed rod-like structure Export

@article{citeulike:487518, abstract = {Incoming herpes simplex virus type-1 (HSV-1) capsids are known to dock to the nuclear pore complex (NPC) and release their genome. It has remained elusive, however, how the huge viral DNA translocates through the comparatively small NPC channel. In the present study, the interaction of HSV-1 with NPCs was analyzed by atomic force microscopy. In addition to capsids, smaller subviral structures - most with a diameter of 35-40 nm and a length of 130-160 nm - were visualized at the cytoplasmic side of the NPC. These components differed from capsids in their adhesion and stiffness properties, and were the sole subviral structures translocated through dilated NPCs towards the nucleus. It is presumed that they are the HSV-1 genome, and that a change in NPC conformation allows translocation of this genome as a densely packaged, rodlike structure.}, author = {Shahin, Victor and Hafezi, Wali and Oberleithner, Hans and Ludwig, Yvonne and Windoffer, Barbara and Schillers, Hermann and Kuhn, Joachim E.}, citeulike-article-id = {487518}, citeulike-linkout-0 = {http://dx.doi.org/10.1242/jcs.02705}, citeulike-linkout-1 = {http://jcs.biologists.org/cgi/content/abstract/119/1/23}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/16339172}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=16339172}, day = {1}, doi = {10.1242/jcs.02705}, journal = {J Cell Sci}, keywords = {hsv, virus-entry}, month = {January}, number = {1}, pages = {23--30}, posted-at = {2006-01-31 17:28:07}, priority = {2}, title = {The genome of HSV-1 translocates through the nuclear pore as a condensed rod-like structure}, url = {http://dx.doi.org/10.1242/jcs.02705}, volume = {119}, year = {2006} }

TY - JOUR ID - citeulike:487518 L3 - citeulike-article-id:487518 N2 - Incoming herpes simplex virus type-1 (HSV-1) capsids are known to dock to the nuclear pore complex (NPC) and release their genome. It has remained elusive, however, how the huge viral DNA translocates through the comparatively small NPC channel. In the present study, the interaction of HSV-1 with NPCs was analyzed by atomic force microscopy. In addition to capsids, smaller subviral structures - most with a diameter of 35-40 nm and a length of 130-160 nm - were visualized at the cytoplasmic side of the NPC. These components differed from capsids in their adhesion and stiffness properties, and were the sole subviral structures translocated through dilated NPCs towards the nucleus. It is presumed that they are the HSV-1 genome, and that a change in NPC conformation allows translocation of this genome as a densely packaged, rodlike structure. IS - 1 JF - J Cell Sci EP - 30 TI - The genome of HSV-1 translocates through the nuclear pore as a condensed rod-like structure VL - 119 SP - 23 KW - hsv KW - virus-entry AU - Shahin, Victor AU - Hafezi, Wali AU - Oberleithner, Hans AU - Ludwig, Yvonne AU - Windoffer, Barbara AU - Schillers, Hermann AU - Kuhn, Joachim PY - 2006/01/01/ UR - http://dx.doi.org/10.1242/jcs.02705 ER -