Yeast life span extension by depletion of 60s ribosomal subunits is mediated by Gcn4. Export

@article{citeulike:2711126, abstract = {In nearly every organism studied, reduced caloric intake extends life span. In yeast, span extension from dietary restriction is thought to be mediated by the highly conserved, nutrient-responsive target of rapamycin (TOR), protein kinase A (PKA), and Sch9 kinases. These kinases coordinately regulate various cellular processes including stress responses, protein turnover, cell growth, and ribosome biogenesis. Here we show that a specific reduction of 60S ribosomal subunit levels slows aging in yeast. Deletion of genes encoding 60S subunit proteins or processing factors or treatment with a small molecule, which all inhibit 60S subunit biogenesis, are each sufficient to significantly increase replicative life span. One mechanism by which reduced 60S subunit levels leads to life span extension is through induction of Gcn4, a nutrient-responsive transcription factor. Genetic epistasis analyses suggest that dietary restriction, reduced 60S subunit abundance, and Gcn4 activation extend yeast life span by similar mechanisms.}, address = {Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.}, author = {Steffen, K. K. and MacKay, V. L. and Kerr, E. O. and Tsuchiya, M. and Hu, D. and Fox, L. A. and Dang, N. and Johnston, E. D. and Oakes, J. A. and Tchao, B. N. and Pak, D. N. and Fields, S. and Kennedy, B. K. and Kaeberlein, M.}, citeulike-article-id = {2711126}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.cell.2008.02.037}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/18423200}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=18423200}, citeulike-linkout-3 = {http://www.sciencedirect.com/science/article/B6WSN-4S9G1SD-H/1/10bf3ac2e90da557944e2b7c9a0cbf8e}, day = {18}, doi = {10.1016/j.cell.2008.02.037}, issn = {1097-4172}, journal = {Cell}, keywords = {cellcycle, complex, deletion, gene, yeast}, month = {April}, number = {2}, pages = {292--302}, posted-at = {2008-07-10 09:28:01}, priority = {2}, title = {Yeast life span extension by depletion of 60s ribosomal subunits is mediated by Gcn4.}, url = {http://dx.doi.org/10.1016/j.cell.2008.02.037}, volume = {133}, year = {2008} }

TY - JOUR ID - citeulike:2711126 L3 - citeulike-article-id:2711126 N2 - In nearly every organism studied, reduced caloric intake extends life span. In yeast, span extension from dietary restriction is thought to be mediated by the highly conserved, nutrient-responsive target of rapamycin (TOR), protein kinase A (PKA), and Sch9 kinases. These kinases coordinately regulate various cellular processes including stress responses, protein turnover, cell growth, and ribosome biogenesis. Here we show that a specific reduction of 60S ribosomal subunit levels slows aging in yeast. Deletion of genes encoding 60S subunit proteins or processing factors or treatment with a small molecule, which all inhibit 60S subunit biogenesis, are each sufficient to significantly increase replicative life span. One mechanism by which reduced 60S subunit levels leads to life span extension is through induction of Gcn4, a nutrient-responsive transcription factor. Genetic epistasis analyses suggest that dietary restriction, reduced 60S subunit abundance, and Gcn4 activation extend yeast life span by similar mechanisms. IS - 2 JF - Cell SN - 1097-4172 AD - Department of Biochemistry, University of Washington, Seattle, WA 98195, USA. EP - 302 TI - Yeast life span extension by depletion of 60s ribosomal subunits is mediated by Gcn4. VL - 133 SP - 292 KW - cellcycle KW - complex KW - deletion KW - gene KW - yeast AU - Steffen, KK AU - MacKay, VL AU - Kerr, EO AU - Tsuchiya, M AU - Hu, D AU - Fox, LA AU - Dang, N AU - Johnston, ED AU - Oakes, JA AU - Tchao, BN AU - Pak, DN AU - Fields, S AU - Kennedy, BK AU - Kaeberlein, M PY - 2008/04/18/ UR - http://dx.doi.org/10.1016/j.cell.2008.02.037 ER -