High-Quality Binary Protein Interaction Map of the Yeast Interactome Network Export

@article{citeulike:3147104, abstract = {Current yeast interactome network maps contain several hundred molecular complexes with limited and somewhat controversial representation of direct binary interactions. We carried out a comparative quality assessment of current yeast interactome data sets, demonstrating that high-throughput yeast two-hybrid (Y2H) screening provides high-quality binary interaction information. Because a large fraction of the yeast binary interactome remains to be mapped, we developed an empirically controlled mapping framework to produce a "second-generation" high-quality, high-throughput Y2H data set covering [\~{}]20\% of all yeast binary interactions. Both Y2H and affinity purification followed by mass spectrometry (AP/MS) data are of equally high quality but of a fundamentally different and complementary nature, resulting in networks with different topological and biological properties. Compared to co-complex interactome models, this binary map is enriched for transient signaling interactions and intercomplex connections with a highly significant clustering between essential proteins. Rather than correlating with essentiality, protein connectivity correlates with genetic pleiotropy. 10.1126/science.1158684}, author = {Yu, Haiyuan and Braun, Pascal and Yildirim, Muhammed A. and Lemmens, Irma and Venkatesan, Kavitha and Sahalie, Julie and Hirozane-Kishikawa, Tomoko and Gebreab, Fana and Li, Na and Simonis, Nicolas and Hao, Tong and Rual, Jean-Francois and Dricot, Amelie and Vazquez, Alexei and Murray, Ryan R. and Simon, Christophe and Tardivo, Leah and Tam, Stanley and Svrzikapa, Nenad and Fan, Changyu and de Smet, Anne-Sophie and Motyl, Adriana and Hudson, Michael E. and Park, Juyong and Xin, Xiaofeng and Cusick, Michael E. and Moore, Troy and Boone, Charlie and Snyder, Michael and Roth, Frederick P. and Barabasi, Albert-Laszlo and Tavernier, Jan and Hill, David E. and Vidal, Marc}, citeulike-article-id = {3147104}, citeulike-linkout-0 = {http://dx.doi.org/10.1126/science.1158684}, citeulike-linkout-1 = {http://www.sciencemag.org/cgi/content/abstract/1158684//}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/18719252}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=18719252}, day = {3}, doi = {10.1126/science.1158684}, issn = {1095-9203}, journal = {Science}, keywords = {interaction, network, protein, yeast}, month = {October}, number = {5898}, pages = {104--110}, posted-at = {2008-10-15 08:39:36}, priority = {4}, title = {High-Quality Binary Protein Interaction Map of the Yeast Interactome Network}, url = {http://dx.doi.org/10.1126/science.1158684}, volume = {322}, year = {2008} }

TY - JOUR ID - citeulike:3147104 L3 - citeulike-article-id:3147104 N2 - Current yeast interactome network maps contain several hundred molecular complexes with limited and somewhat controversial representation of direct binary interactions. We carried out a comparative quality assessment of current yeast interactome data sets, demonstrating that high-throughput yeast two-hybrid (Y2H) screening provides high-quality binary interaction information. Because a large fraction of the yeast binary interactome remains to be mapped, we developed an empirically controlled mapping framework to produce a "second-generation" high-quality, high-throughput Y2H data set covering [~]20% of all yeast binary interactions. Both Y2H and affinity purification followed by mass spectrometry (AP/MS) data are of equally high quality but of a fundamentally different and complementary nature, resulting in networks with different topological and biological properties. Compared to co-complex interactome models, this binary map is enriched for transient signaling interactions and intercomplex connections with a highly significant clustering between essential proteins. Rather than correlating with essentiality, protein connectivity correlates with genetic pleiotropy. 10.1126/science.1158684 IS - 5898 JF - Science SN - 1095-9203 EP - 110 TI - High-Quality Binary Protein Interaction Map of the Yeast Interactome Network VL - 322 SP - 104 KW - interaction KW - network KW - protein KW - yeast AU - Yu, Haiyuan AU - Braun, Pascal AU - Yildirim, Muhammed AU - Lemmens, Irma AU - Venkatesan, Kavitha AU - Sahalie, Julie AU - Hirozane-Kishikawa, Tomoko AU - Gebreab, Fana AU - Li, Na AU - Simonis, Nicolas AU - Hao, Tong AU - Rual, Jean-Francois AU - Dricot, Amelie AU - Vazquez, Alexei AU - Murray, Ryan AU - Simon, Christophe AU - Tardivo, Leah AU - Tam, Stanley AU - Svrzikapa, Nenad AU - Fan, Changyu AU - de Smet, Anne-Sophie AU - Motyl, Adriana AU - Hudson, Michael AU - Park, Juyong AU - Xin, Xiaofeng AU - Cusick, Michael AU - Moore, Troy AU - Boone, Charlie AU - Snyder, Michael AU - Roth, Frederick AU - Barabasi, Albert-Laszlo AU - Tavernier, Jan AU - Hill, David AU - Vidal, Marc PY - 2008/10/03/ UR - http://dx.doi.org/10.1126/science.1158684 ER -