The mouse Pol I terminator is more efficient than the hepatitis delta virus ribozyme in generating influenza-virus-like RNAs with precise 3′ ends in a plasmid-only-based virus rescue system Export

@article{citeulike:4875709, abstract = {Abstract\ \ Reverse genetics systems for generating recombinant influenza viruses are based on two different mechanisms for obtaining the 3′ end of the viral RNA: one uses the self-cleaving hepatitis delta virus ribozyme (HDVR), and the other uses the murine RNA polymerase I (Pol I) terminator. In this study, we employed EGFP and Renilla luciferase reporter constructs to compare the efficiency of both methods. Our results indicate that the murine Pol I terminator was more efficient than the HDVR, which will be helpful in choosing an influenza virus rescue system, as well as in establishing other RNA virus rescue systems.}, author = {Feng, Liqiang and Li, Feng and Zheng, Xuehua and Pan, Weiqi and Zhou, Kai and Liu, Yichu and He, Hongxuan and Chen, Ling}, citeulike-article-id = {4875709}, citeulike-linkout-0 = {http://dx.doi.org/10.1007/s00705-009-0422-9}, citeulike-linkout-1 = {http://www.springerlink.com/content/e81112p45761571g}, doi = {10.1007/s00705-009-0422-9}, journal = {Archives of Virology}, keywords = {influenza, promoter, rna, terminator}, posted-at = {2009-06-17 02:25:16}, priority = {4}, title = {The mouse Pol I terminator is more efficient than the hepatitis delta virus ribozyme in generating influenza-virus-like RNAs with precise 3′ ends in a plasmid-only-based virus rescue system}, url = {http://dx.doi.org/10.1007/s00705-009-0422-9} }

TY - JOUR ID - citeulike:4875709 L3 - citeulike-article-id:4875709 N2 - Abstract  Reverse genetics systems for generating recombinant influenza viruses are based on two different mechanisms for obtaining the 3′ end of the viral RNA: one uses the self-cleaving hepatitis delta virus ribozyme (HDVR), and the other uses the murine RNA polymerase I (Pol I) terminator. In this study, we employed EGFP and Renilla luciferase reporter constructs to compare the efficiency of both methods. Our results indicate that the murine Pol I terminator was more efficient than the HDVR, which will be helpful in choosing an influenza virus rescue system, as well as in establishing other RNA virus rescue systems. JF - Archives of Virology TI - The mouse Pol I terminator is more efficient than the hepatitis delta virus ribozyme in generating influenza-virus-like RNAs with precise 3′ ends in a plasmid-only-based virus rescue system KW - influenza KW - promoter KW - rna KW - terminator AU - Feng, Liqiang AU - Li, Feng AU - Zheng, Xuehua AU - Pan, Weiqi AU - Zhou, Kai AU - Liu, Yichu AU - He, Hongxuan AU - Chen, Ling UR - http://dx.doi.org/10.1007/s00705-009-0422-9 ER -